MIP-3α/CCL20

CRP08115

Recombinant Human MIP-3 alpha/CCL20 produced in E. coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 8.0 kDa.

MIP-3 alpha/CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. MIP-3 alpha is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors.

>97% by SDS-PAGE and HPLC analyses.

Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized from a 0.2μm filtered concentrated (0.5mg/ml) solution in 20mM PB, pH 7.4, 100mM NaCl.

Fully biologically active when compared to standard. Determined by its ability to chemoattract human T lymphocytes using a concentration range of 10.0 -50.0 ng/ml.

Less than 1EU/μg of rHuMIP-3a/CCL20 as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

CCL20

Synonyms

CKb4; LARC; exodus-1; MIP-3-alpha ; ST38; MIP3A; MIP-3a; SCYA20; Beta chemokine exodus-1; C-C motif chemokine 20 precursor; chemokine (C-C motif) ligand 20; CC chemokine LARC; CCL20_HUMAN;  Human mRNA for CC chemokine LARC precursor, complete cds; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; Small-inducible cytokine A20; chemokine (C-C motif) ligand 20; small inducible cytokine subfamily A (Cys-Cys), member 20.

GeneID

6364

mRNA Refseq

NM_001130046

Protein Refseq

NP_001123518

MIM

601960

UniProt ID

P78556

Chromosome Location

2q33-q37

Pathway

Cytokine-cytokine receptor interaction.

Function

chemokine activity.