MIP-5/CCL15

CRP08107

Recombinant Human MIP-5/CCL15 produced in E. coli is a single, non-glycosylated polypeptide chain containing 92 amino acids and having a molecular mass of 10.1 kDa.

CCL15, a new human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 amino acids that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1γ, respectively. The gene for MIP-5 is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-5 is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

>97% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 100mM NaCl.

Fully biologically active when compared to standard. Determined by its ability to chemoattract human T lymphocytes using a concentration range of 1.0 -10.0 ng/ml.

Less than 1EU/mg of rHuMIP-5/CCL15 as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

CCL15

Synonyms

LKN1;NCC3;SY15;HCC-2;Lkn-1;MIP-5;NCC-3; SCYL3;MIP-1d;SCYA15;HMRP-2B;chemokine (C-C motif) ligand 15; CCL15_HUMAN; MIP5; C-C motif chemokine 15 [Precursor]; Small-inducible cytokine A15;Macrophage inflammatory protein 5;MIP-5;Chemokine CC-2; MIP-1delta; Chemokine CC-2;Leukotactin-1;Mrp-2b; small inducible cytokine subfamily A (Cys-Cys), member 15.

GeneID

6359

mRNA Refseq

NM_004167

Protein Refseq

NP_004158

MIM

601393

UniProt ID

Q16663

Chromosome Location

17q11.2

Pathway

Cytokine-cytokine receptor interaction

Function

chemoattractant activity;chemokine activity; heparin binding; heparin binding; signal transducer activity.