NAP-2/CXCL7

CRP08108

Recombinant Human NAP-2/CXCL7 produced in E. coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 7.6 kDa.

Neutrophil Activating Peptide 2 (NAP-2) is proteolytically processed carboxyl-terminal fragments of platelet basic protein (PBP) which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines. Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR-2 and to chemoattract and activate neutrophils. Although CTAP-III, β-TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. Recently, it has been shown that the additional amino-terminal residues of CTAP-III masks the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity.

>97% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.

Fully biologically active when compared to standard. Determined by its ability to chemoattract human neutrophils using a concentration range of 1.0-10.0 ng/ml.

Less than 1EU/mg of rHuNAP-2/CXCL7 as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

PPBP

Synonyms

PBP; TC1; TC2; TGB; LDGF; MDGF; TGB1; B-TG1; CTAP3; CXCL7; NAP-2; SCYB7; THBGB; LA-PF4; THBGB1; Beta-TG; CTAPIII; NAP-2-L1; TGB1;SCYB7; CXCL7_HUMAN; Platelet basic protein [Precursor]; C-X-C motif chemokine 7;Small-inducible cytokine B7;Leukocyte-derived growth factor;LDGF;Macrophage-derived growth factor;MDGF.

GeneID

5473

mRNA Refseq

NM_002704

Protein Refseq

NP_002695

MIM

121010

UniProt ID

P02775

Chromosome Location

4q12-q13

Pathway

Cytokine-cytokine receptor interaction; Leukocyte transendothelial migration; Hemostasis.

Function

chemokine activity;glucose transmembrane transporter activity;growth factor activity.