CRP0892

Recombinant human OPG/Fc produced in Pichia. P. contains 412 amino acid residues, including 180 residues from mature OPG (a.a 22-201) and 232 residues from the Fc protein of human IgG1, and has a calculated molecular mass of 46.5 kDa. As a result of glycosylation, the recombinant OPG/Fc migrates as a 49 kDa protein in SDS-PAGE under reducing conditions.

Osteoprotegerin (OPG) is a member of the TNFR superfamily that can act as a decoy receptor for RANKL. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. OPG is expressed in a wide variety of tissues including adult heart, lung, kidney, liver, spleen, prostate, lymph node and bone marrow. OPG is secreted both as a monomeric and a dimeric protein. Its primary structure consists of seven distinct domains, four of which corresponds to the extracellular cysteine-rich domains of TNFR proteins and constitutes the soluble OPG.

>90% by SDS-PAGE and HPLC analyses.

Fully biologically active when compared to the standard. Determined by its ability to neutralize the stimulation of U937 cells treated with 10 ng/ml of soluble RANKL.

Lyophilized from a 0.2μm filtered concentrated (0.5mg/ml) solution in PBS, pH 7.4.

Less than 1EU/μg of rHuOPG-Fc as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

TNFRSF11B

Synonyms

OPG; TR1; OCIF; MGC29565; Osteoprotegerin; osteoclastogenesis inhibitory factor; Tumor necrosis factor receptor superfamily member 11B; Tumor necrosis factor receptor superfamily member 11B Precursor

GeneID

4982

mRNA Refseq

NM_002546

Protein Refseq

NP_002537

MIM

602643

UniProt ID

O00300

Chromosome Location

8q24

Pathway

Cytokine-cytokine receptor interaction

Function

cytokine activity; protein binding; receptor activity