OSM

CRP0893

Recombinant Human Oncostatin M produced in E. coli is a single non-glycosylated polypeptide chain containing 227 amino acids and having a molecular mass of 26kDa.

Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi's sarcoma cells, OSH can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi's sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptor in hepatoma cells. OSM share several structural and functional characteristics with LIF, IL-6, and CNTF. Human OSM is active on murine cells.

>95% by SDS-PAGE and HPLC analyses.

Fully biologically active when compared to the standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is < 2 ng/ml, corresponding to a specific activity of > 5×105 units/mg.

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in PBS, pH 7.4.

Less than 1EU/mg of rHuOSM as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤20°C. Further dilutions should be made in appropriate buffered solution

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

OSM

Synonyms

MGC20461; Oncostatin-M; Oncostatin-M Precursor

GeneID

5008

mRNA Refseq

NM_020530

Protein Refseq

NP_065391

MIM

165095

UniProt ID

P13725

Chromosome Location

22q12.2

Pathway

Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway

Function

cytokine activity; oncostatin-M receptor binding