PEDF

 Cat. No.

CRP0870

 Product Overview

Recombinant Human Pigment Epithelium-derived Factor produced in E.coli is a single non-glycosylated polypeptide chain containing 400 amino acids and having a molecular mass of approximately 44.5KDa.

 Description

PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is a 50 kDa glycoprotein produced and secreted in many tissues throughout the body. A major component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. In addition, PEDF is able to inhibit the activity of angiogenic factors such as VEGF and FGF-2. The neuroprotective effects of PEDF are achieved through suppression of neuronal apoptosis induced by peroxide, glutamate, or other neurotoxins. The recent identification of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. To date, PEDF-R is the only signaling receptor known to be used by a serpin family member.

 Purity

>95% by SDS-PAGE and HPLC analyses.

 Formulation

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH7.4, 150mM NaCl.   

 Endotoxin

Less than 1EU/mg of rHuPEDF as determined by LAL method

 Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.   

 Storage

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.   

 Gene Information

Gene Name

SERPINF1

Synonyms

EPC-1, PEDF, PIG35, Serpin-F1; pigment epithelium-derived factor; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; Pigment epithelium-derived factor precursor; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1

GeneID

5176

mRNA Refseq

NM_002615

Protein Refseq

NP_002606

MIM

172860

Unitprot ID

P36955

Chromosome Location

17p13.1

Function

serine-type endopeptidase inhibitor activity