|
Cat. No. |
CRP0816 |
| Product Overview |
Recombinant Human Interferon alpha-2b produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19269 Dalton. Pegylated IFN alpha-2b is manufactured by attaching a 40KD NHS-mPEG to the primary amino acid of IFN alpha-2b. |
| Description |
At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. |
| Purity |
>98% as determined by SDS-PAGE , RP-HPLC and FPLC. |
| Formulation |
Lyophilized from a (0.5mg/ml) solution in containing 8.8mg sodium chloride, 0.4mg sodium acetate and 1.0mg acetate acid buffer. |
| Specific Activity |
The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be 1.0 x 106 IU/ mg. |
| Endotoxin |
Less than 0.1 ng/ug (IEU/ug) of PEG-IFN alpha-2b. |
| Reconstitution |
It is recommended to reconstitute the lyophilized PEG-IFN alpha-2b in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Storage |
Lyophilized PEG-IFN alpha-2b although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution PEG-IFN alpha-2b should be stored at 4oC between 2-7 days and for future use below -18oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles. |
| Gene Information |
Gene Name |
IFNA2 |
|
Synonyms |
IFNA, INFA2, MGC125764, MGC125765, Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-α 2a, Interferon alpha-A, LeIF A, Interferon alpha-2; Interferon alpha-2 Precursor. |
|
GeneID |
3440 |
|
mRNA Refseq |
NM_000605.2 |
|
Protein Refseq |
NP_000596.2 |
|
MIM |
147562 |
|
UniProt ID |
P01563 |
|
Chromosome Location |
9p22 |
| Pathway |
Antigen processing and presentation; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; KEGG pathway: Natural killer cell mediated cytotoxicity; Regulation of autophagy; Toll-like receptor signaling pathway |
| Function |
Interferon-alpha/beta receptor binding |
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