PTH7-34

CRP0865

Recombinant Human Parathyroid Hormone 7-34 produced in E. coli is a single, non-glycosylated Polypeptide chain containing  28 amino acids and having a molecular mass of 3380 Da.

Polypeptide hormones secreted by the parathyroid glands, which promote release of calcium from bone to extracellular fluid by activating osteoblasts and inhibiting osteoclasts, indirectly promote increased intestinal absorption of calcium, and promote renal tubular reabsorption of calcium and increased renal excretion of phosphates. It is a major regulator of bone metabolism. Secretion of parathyroid hormone increases when the level of calcium in the extracellular fluid is low. Its action is opposed by calcitonin. PTH (7-34), which is a PTH/PTHrP receptor antagonist, can stimulate hair growth and epidermal proliferation in mice.

1.0 mg

>97% by SDS-PAGE and HPLC analyses.

Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in 20mM PB, pH 7.0, containing 4% mannitol.

Fully biologically active when compared to standard. The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 1.0 x 104 Units/mg.

Less than 1EU/mg of rHuPTH7-34 as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Gene Name

PTH

Synonyms

parathormone; parathyrin; Parathyroid hormone precursor; parathyroid hormone

GeneID

5741

mRNA Refseq

NM_000315

Protein Refseq

NP_000306

MIM

168450

UniProt ID

P01270

Chromosome Location

11p15.3-p15.1

Pathway

Neuroactive ligand-receptor interaction

Function

hormone activity; parathyroid hormone receptor binding activity