Recombinant Human SCF produced in E. coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton.

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.

rHuSCF was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM Acetic acid.

The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is less than 2 ng/ml, corresponding to a Specific Activity of 5.0 x 10⁵ IU/ mg.

Less than 0.1 ng/ug (IEU/ug) of rHuSCF.

It is recommended to reconstitute the lyophilized rHuSCF in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Lyophilized rHuSCF although stable at room temperature for 3 weeks, should be stored desiccated below -18^oC. Upon reconstitution rHuSCF should be stored at 4^oC between 2-7 days and for future use below -18^oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.

KL-1, Kitl, MGF, SCF, SF, SHEP7, C-kit ligand, Stem cell factor, Mast cell growth factor, Kit ligand; Kit ligand Precursor

Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Melanogenesis

growth factor activity; identical protein binding; stem cell factor receptor binding