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 Cat. No.

CRP0860
 Product Overview

Recombinant Human Superoxide Dismutase produced in E.coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 154 amino acids and having a molecular mass of approximately 31 kDa.
 Description

Human Cu/Zn Superoxide Dismutase (SOD) catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide.
 Purity

>85% by SDS-PAGE and HPLC analyses.
 Formulation

Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
 Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.
 Specific Activity

Fully biologically active when compared to standard. The potency per mg was tested by Pyrogallic Acid method and was found to be more than 3,000Units/mg.
 Endotoxin

Less than 1EU/μg of rHuSOD as determined by LAL method.
 Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20oC. Further dilutions should be made in appropriate buffered solutions.
 Storage

This lyophilized preparation is stable for several weeks at 2-8oC, but should be kept at -20o for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20oC to -70oC. Avoid repeated freeze/thaw cycles.
 Gene Information

 

Gene Name

SOD1 superoxide dismutase 1, soluble [ Homo sapiens ]

Synonyms

SOD1; superoxide dismutase 1, soluble; ALS; SOD; ALS1; IPOA; homodimer; SOD, soluble; indophenoloxidase A; Cu/Zn superoxide dismutase; superoxide dismutase, cystolic; EC 1.15.1.1; Superoxide dismutase [Cu-Zn]

GeneID

6647

mRNA Refseq

NM_000454

Protein Refseq

NP_000445

Enzyme Commission Number

EC 1.15.1.1

MIM

147450

UniProt ID

P00441

Chromosome Location

21q22.1; 21q22.11

Pathway

Amyotrophic lateral sclerosis (ALS); Huntington's disease; Prion diseases; Hemostasis

Function

antioxidant activity; chaperone binding; copper ion binding; metal ion binding; oxidoreductase activity; protein homodimerization activity; protein phosphatase 2B binding; superoxide dismutase activity; zinc ion binding