CRP08135

Recombinant Human Ubiquitin Conjugating Enzyme 7 produced in E.coli is a single non-glycosylated polypeptide chain containing 162 amino acids and having a molecular mass of approximately 18.9 kDa.

Human Ubquitin Conjugating Enzyme 7 (UbcH7) is a class I enzyme which functions in the stress response and the control of transcription factors. The enzyme is ubiquitously expressed with high levels of expression seen in adult muscle. UbcH7 mediates the selective degradation of short-lived and abnormal proteins and is highly homologous to UbcH5. It has been demonstrated to participate in the ubiquitinylation of p53, c-Fos and NF-κB. UbcH7 is one of two E2s (UbcH5 being the other) with which HECT domain proteins interact with UbcH7 being able to efficiently substitute for UbcH5 in E6-AP-dependent ubiquitinylation.

Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in 1×PBS, 1mM DTT, pH 7.5.

>95% by SDS-PAGE and HPLC analyses.

Less than 1EU/μg of rHuUbcH7 as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

UBE2L3

Synonyms

E2-F1; L-UBC; UBCH7; UbcM4; ubiquitin-conjugating enzyme E2L 3; UB2L3_HUMAN; EC 6.3.2.19; Ubiquitin-protein ligase L3;Ubiquitin carrier protein L3; UBCE7; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2L3; ubiquitin-conjugating enzyme UBCH7; ubiquitin-protein ligase.

GeneID

7332

mRNA Refseq

NM_003347

Protein Refseq

NP_003338

MIM

603721

UniProt ID

P68036

Chromosome Location

22q11.21

pathway

Parkinson's disease

Function

enzyme binding; ligase activity; small conjugating protein ligase activity; ubiquitin-protein ligase activity.