CRP08102

Recombinant murine VEGF165 produced in E. coli is a 39.0 kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.

VEGF was initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and by a variety of transformed cell lines as a mitogen specific for vascular endothelial cells. It was subsequently found to be identical to an independently discovered vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based on its ability to increase the permeability of capillary blood vessels. Three mouse cDNA clones, which arise through alternative splicing and which encode mature mouse monomeric VEGF having 120, 164, or 188, amino acids, respectively, have been identified. Two receptor tyrosine kinases (RTKs), Flt-1 and Flk-1 (the mouse homologue of human KDR), both members of the type III subclass of RTKs containing seven immunoglobulin-like repeats in their extracellular domains, have been shown to bind VEGF with high affinity. The roles of the homodimers of KDR, Flt, and the heterodimer ofKDR/Flt in VEGF signal transduction remain to be elucidated.In vivo, VEGF has been found to be a potent angiogenesis inducer.

>95% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.

Less than 1EU/mg of rmVEGF165 as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

VEGF165

Synonyms

Vpf; Vegf; Vegf-a; Vegfa; Vegf120; Vegf164; Vegf188; vascular endothelial growth factor; vascular endothelial growth factor A; MGC70609; Vascular endothelial growth factor A precursor; Vascular permeability factor

GeneID

22339

mRNA Refseq

NM_001025250

Protein Refseq

NP_001020421

MIM

192240

UniProt ID

P15692

Chromosome Location

17 C; 17 24.2 cM

Pathway

Cytokine-cytokine receptor interaction;Focal adhesion ;Pancreatic cancer;Renal cell carcinoma;VEGF signaling pathway;mTOR signaling pathway .

Function

growth factor activity; heparin binding; protein binding growth factor receptor binding.