bEK

CRP08116

Recombinant Bovine Enterokinase produced in Pichia. Pastoris is a single glycosylated polypeptide chain containing 235 amino acids and having a molecular mass of approximately 43.0 kDa.

Enterokinase is a serine proteinase in the duodenum that plays a critical role in mammalian digestion. It is the physiological activator of pancreatic trypsinogen. It converts trypsinogen into its active form trypsin, by cleaving its aminoterminal hexapeptide Val(Asp)4-Lys. More recently, the enterokinase has been shown to have a broad utility in cleaving fusion proteins produced in Escherichia coli. The enzyme is particularly suitable for this role because of its high degree of specificity, its tolerance to a wide range of reaction conditions, and the fact that its recognition sequence lies entirely on the aminoterminal side of the scissile bond. This enzymatic activity allows release of carboxyl-terminal fusion partners from fusion proteins without leaving unwanted amino acid residues on their amino termini.

One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2.

50 mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.

Sterile liquid

Less than 1EU/mg of rbEK as determined by LAL method.

One year when stored at -20°C. Avoid repeated freeze/thaw cycles.

Gene Name

protease, serine, 7 (enterokinase)

Synonyms

PRSS7; EC 3.4.21.9; ENTK; Enterokinase; MGC133046; enterokinase; enteropeptidase; proenterokinase; Enteropeptidase precursor; Serine protease 7; human enterokinase;EC 3.4.21.9; protease, serine, 7; serine protease (enterokinase).

GeneID

282009

mRNA Refseq

NM_174439

Protein Refseq

NP_776864

Enzyme Commission Number

EC 3.4.21.9

UniProt ID

P98072

Chromosome Location

1

Reaction

The reaction catalysed by Enteropeptidase:

trypsinogen → trypsin + octapeptide

Enteropeptidase cleaves after Lysine if the Lys is preceded by four Asp and not followed by a Pro.

Function

peptidase activity;scavenger receptor activity