Nanodiscs

The nanodiscs is a non-covalent structure of lipid bilayer and membrane scaffold protein (MSP). MSPs are genetically engineered proteins, derived from human apolipoprotein A-1 to shield the hydrophobic core of the lipids from the aqueous phase. The size of the nanodiscs was controlled by the length of the MSPs. The diameter of nanodiscs is typically in the order of ~10 nm, but generation of specific MSP variants allows the formation of smaller (6-7 nm) and larger (16-17 nm) nanodiscs. Nanodiscs is useful in the study of membrane proteins and is a more native environment than liposomes and detergent micelles.

Membrane Scaffold Proteins (MSPs)

MSPs are typically used for the reconstitution of already isolated proteins. Creative Biolabs offers a variety of MSPs that can be assembled into nanodiscs of different sizes.

Cat	Product Name	Species	Tag	Nanodiscs Size
MPN0001K	MSP1E3D1, His-	Streptomyces kanamyceticus	His tag at N-terminus	12.9 nm in diameter
MPN0002K	MSP1D1, His-	Microbial	His tag at N-terminus	9.7 nm in diameter
MPN0016K	MSP1D1 ∆H5, tag-free	Human	Tag-free	7-8 nm in diameter
MPN0017K	MSP1D1, tag-free	Mouse	Tag-free	9-10 nm in diameter
MPN0024K	MSP1E3D1, tag-free	Human	Tag-free	12-14 nm in diameter
MPN0025K	MSP2N2, His	Human	His tag	17 nm in diameter
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Assembled Nanodiscs

Creative Biolabs also offers assembled nanodiscs used in cell-free protein expression. Assembled nanodiscs aren't recommended for the reconstitution of membrane proteins.

Cat	Product Name	Species	Tag	Phospholipids	Nanodiscs Size
MPN0035K	MSP1D1-His with DMPC	Human	His tag	DMPC	9-10 nm in diameter
MPN0036K	MSP1D1-His with POPC	Human	His tag	POPC	9-10 nm in diameter
MPN0037K	MSP1D1-His with DMPG	Human	His tag	DMPG	9-10 nm in diameter
MPN0041K	MSP1D1-tag-free with DMPC	Human	Tag-free	DMPC	9-10 nm in diameter
MPN0042K	MSP1D1-tag-free with POPC	Human	Tag-free	POPC	9-10 nm in diameter
MPN0043K	MSP1D1-tag-free with DMPG	Human	Tag-free	DMPG	9-10 nm in diameter
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Nanodiscs Assembly Kit

Creative Biolabs provides various Nanodiscs Assembly Kit with different combinations of MSPs and phospholipids. The kits include MSP protein, phospholipids, and sodium cholate They can assemble into nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains.

Cat	Product Name	Species	Tag	Phospholipids	Nanodiscs Size
MPN0079K	MSP2N2-His with DMPC Nanodisc Assembly Kit	Human	His tag	DMPC	17 nm in diameter
MPN0080K	MSP2N2-His with POPC Nanodisc Assembly Kit	Human	His tag	POPC	17 nm in diameter
MPN0081K	MSP1D1-His with DMPC Nanodisc Assembly Kit	Human	His tag	DMPC	9-10 nm in diameter
MPN0082K	MSP1D1-His with POPC Nanodisc Assembly Kit	Human	His tag	POPC	9-10 nm in diameter
MPN0083K	MSP1D1 ∆H5-His with DMPC Nanodisc Assembly Kit	Human	His tag	DMPC	7-8 nm in diameter
MPN0084K	MSP1D1 ∆H5-His with POPC Nanodisc Assembly Kit	Human	His tag	POPC	7-8 nm in diameter
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Application of Membrane Protein in Nanodiscs Form

Immunization & antibody selection
Studies of protein-protein/protein-lipid/protein-ligand/protein-additive interactions
Membrane protein delivery
Studies of membrane protein conformation dynamics
Discovery of small-molecule drugs
Binding assays (SPR, BLItz, etc.)
Solution-state NMR
Cryo-EM
Single-molecule characterizations (SMFS, TIRFM, etc.)

All listed customized services & products are for research use only, not intended for pharmaceutical, diagnostic, therapeutic or any in vivo human use.

Membrane Protein Solubilization and Stabilization Reagents

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