Magic™ Cell-free Membrane Protein Expression in Human Hela Cell Lysate

Magic™ Cell-free Membrane Protein Expression in Human Hela Cell Lysate Kit is a mammalian in vitro expression system based on HeLa cell lysates. The kit contains all factors required for protein synthesis including ribosomes, initiation factors, elongation factors, and tRNA. Reactions are started by mixing plasmid DNA with the reaction mix. This system can synthesize protein for up to 6 hours.

Magic™ Cell-free Membrane Protein Expression in Human Hela Cell Lysate Kit is an optimized kit contains a T7 promoter and an EMCV internal ribosome entry site (IRES) to facilitate high levels of in vitro membrane protein expression. Using a vector containing the EMCV IRES element is critical for obtaining high expression levels in this human in vitro membrane protein expression system.

Components List and Storage Information

Within the kit, it contains optimized all necessary components, listed in the table below. In Magic™ Cell-free Membrane Protein Expression in Human Hela Cell Lysate Kit, membrane protein expression reactions can easily be set up with a few pipetting steps. Subsequently, analyze or purify the proteins (if needed) or store the proteins at -20°C for further processing or analysis.

Expression System	Components	Storage
In Vitro Human Hela Cell Expression System	HeLa Cell Extract	-80°C
	Accessory Proteins	-80°C
	Reaction Mix	-80°C
	Nuclease-free Water	-80°C
	Positive Control DNA (0.5 µg/µL)	-80°C
	Expression Vector (0.5 µg/µL)	-80°C

Features

High-yield: Membrane protein production up to 100 µg/mL
Easy to use: One-step reaction protocol
Fast: High yield in just 6 hours
Flexible: Express toxic or insoluble proteins, faster protein synthesis and protein labeling with modified amino acids, etc.

Applications

Express membrane proteins to measure enzyme activity
Perform mutational analysis
Express membrane protein for use in gel mobility shift assays
Perform co-immunoprecipitation
Express cytotoxic proteins
Perform unnatural amino acid labeling

Creative Biolabs is a world-leading service provider of cell-free protein production. Moreover, we now provide integrated Magic™ cell-free membrane protein production service in Hela cell lysate for our clients around the world. To get more information, please feel free to contact us.

Related Products

Cat	Product Name	Expected Yield of Positive Control
MPCFK-2-2	Magic™ Cell-free Membrane Protein Expression in Human Hela Cell Lysate	0.1 mg/mL

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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