Magic™ Cell-free PURE Membrane Protein Expression Kit

Recently, cell-free membrane protein expression has become increasingly popular due to its unique features. The majority of cell-free membrane protein expression systems are generally derived from cell extracts, which usually contain nonspecific proteases and nucleases that have negative effects on protein synthesis. In addition, such unpredictable activities may also interfere with downstream assays. Completely free from unwanted/uncharacterized cellular components, the PURE (Protein synthesis Using Recombinant Elements) system introduces a much cleaner background. PURE membrane protein expression is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E. coli translation. With minimal nuclease and protease activity, the PURE membrane protein expression kit preserves the integrity of DNA and RNA templates/complexes and results in membrane proteins that are free of modification and degradation.

General Protocol

Using a positive control template to verify protein synthesis can be useful when unfamiliar with in vitro transcription-translation protocols. Reactions are usually assembled in nuclease-free 0.5 mL microfuge tubes. Add Solution 2 to Solution 1, do not dilute Solution 2 unbuffered. Mix gently and incubate at 37°C for 2 hours in an incubator, some reactions should be incubated for 4 hours total.

Components List and Storage

Expression System	Components	Storage
In Vitro PURE Expression System	PURE solution 1	-80°C
	PURE solution 2	-80°C
	Control Vector	-80°C

Advantages of PURE Membrane Protein Expression

In vitro compartmentalization
Translation and/or membrane protein folding studies
Confirmation of open reading frames
Quickly synthesize sufficient amounts of membrane protein
Examination of the effects of mutations on ORFs
Introduction of unnatural, modified, or labeled amino acids
Identify active domains and functional residues of truncated proteins

Applications

High throughput screening/directed evolution
Synthetic biology
Toxic or difficult to express protein synthesis
Studies on protein folding, activity, and protein-protein interactions

Creative Biolabs offers world-leading cell-free membrane protein synthesis service using the PURE system. Based on our optimized protocol, our unique PURE system can facilitate high-quality protein expression at very competitive price. Furthermore, we offer various types of cell-free membrane protein production services. To get more information, please feel free to contact us.

Related Products

Cat	Product Name	Expected Yield of Positive Control
MPCFK-4	Magic™ Cell-Free PURE Membrane Protein Expression Kit	0.1-0.2 mg/mL

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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