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CreMap™ Alanine Scanning Mutagenesis Mapping Service

For high-throughput and high-resolution epitope discovery, Creative Biolabs has developed an efficient CreMap™ alanine scanning mutagenesis mapping platform.

Site-directed mutagenesis is one of the most important methods of epitope mapping, in which a highly-specified mutation is introduced into a specific sequence of the antigen of interest, and then the mutant antigen is tested for its ability to bind to the corresponding antibody. Failure of binding signifies that a particular amino acid is critical to the functioning of the antigen being tested. Substitution with alanine residues eliminates side-chain interactions without altering the backbone conformation or introducing steric or electrostatic effects, so is often the preferred choice for testing the contribution of specific side-chains while preserving native protein structure.

CreMap™ alanine scanning mutagenesis mapping platform is a high-throughput strategy for mapping both linear and conformational antigen epitopes by evaluating the effects of point mutations across a target protein. Every single residue in the user-specified protein sequence (the candidate antigen) is replaced by alanine one at a time to construct a comprehensive mutant library. And then, the ability of each the variant to bind to the antibody is evaluated in parallel. The failure of the binding means that the mutated amino acid is a key factor in determining the function of the antigen, and therefore, a map for the epitope of the antigen being tested could be constructed. Meanwhile, through the alanine scanning mutagenesis mapping service, customers can obtain valuable and precise information on the structure-function relationship of target proteins at the level of amino acid. Moreover, with our extensive experience and rich expertise in protein expressions, CreMap™ alanine scanning mutagenesis mapping platform can also be used for the study of challenging targets, such as oligomers, post-translationally modified proteins, and membrane proteins. We will discuss specific projects with each customer, set up reasonable and economic experimental strategies, and optimize each experimental step, to obtain satisfactory results.

General Workflow of CreMapAlanine Scanning Mutagenesis Mapping Service

Features of CreMap Alanine Scanning Mutagenesis Mapping Service

Applications of CreMap Alanine Scanning Mutagenesis Mapping Service

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