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CreMap™ Peptide/Protein Microarray Service

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Creative Biolabs offers a unique and personalized CreMap™ peptide/protein microarrays service for your high-throughput protein interaction screening and epitope identification. Peptide microarrays-based epitope mapping uses a library of oligopeptide sequences from overlapping and non-overlapping segments of a target protein and tests for their ability to bind the antibody of interest. This method is fast and relatively inexpensive, and specifically suited to profile epitopes for a large number of candidate antibodies against a defined target.

In general, this microarrays epitope mapping analysis procedure involves four major steps, which are the synthesis of peptide library, then the use of ELISA as a method for binding analysis, followed by bioinformatics-based data analysis, and finally constructing initial results. Our platform achieves full compatibility for overlapping peptide libraries and proteins and utilizes a flexible peptide synthesis technology to enable printing of the peptide arrays optimized for specific applications. Furthermore, our unique technology ensures greater uniformity of spot morphology between proteins and peptides, reduces background signal and improves assay performance in sensitivity, dynamic range, and variance.

In principle, CreMap™ peptide/protein microarray service works similarly to ELISA and other ligand binding assays. However, it can detect tens of thousands of interactions in a single run and enables higher sensitivity with lower cost. Additionally, it allows various set-up options as per plate-based ligand binding assay for direct assays, sandwich assays, bridging assays, etc. Moreover, your established assay protocols can be easily transferred to our service platform, and what you should supply are only the protein/antibodies/sera. More importantly, each run of microarray analysis only requires microliter amounts of sample which are simply impossible to carry out with 96-well plate-based assays.

Various Applications of CreMap™ Peptide/Protein Microarray Service


Brief Workflow of Our CreMap™ Peptide/Protein Microarray Service


Fig.1 Principle of microarray technology. Fig.1 Principle of microarray technology.

Reference

  1. Chandra K, et al. “Protein microarray with novel microfluidic methods: current advances. Microarrays 2014 (3): 180-202.



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