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CreMap™ T Cell Epitope Discovery Service

T cell epitopes are peptide fragments that presented on the surface of an antigen-presenting cell, where they are bound to MHC molecules. In humans, professional antigen-presenting cells are specialized to present MHC class II peptides, whereas most nucleated somatic cells present MHC class I peptides. T cell epitopes presented by MHC class I molecules are typically peptides between 8 and 11 amino acids in length, whereas MHC class II molecules present longer peptides, 13-17 amino acids in length. The recognition of T cell receptor (TCR) to T cell epitopes presented on MHC I or MHC II molecules could lead to the activation of the adaptive immune system, which is essential for immune responses of infections, diseases, cancers and other pathogens. Thereby, it is of great importance to know which peptide fragment of the pathogenic proteins or protein drug could bind to MHC and then stimulate the immune response in the field of disease treatment, drug development or vaccine design.

However, T cell epitope discovery is a laborious process and need a huge workload. From prediction of candidate epitopes from a lot of potential peptide sequences to identification of the accurate epitope by MHC-peptide binding assay, scientists should take a lot of time to accomplish the whole protocol.

There are several methods that can be applied to T cell epitope discovery, from in silico prediction to in vitro binding analysis. Through bioinformatics and computational biology, one could simulate the interaction and binding mode between the peptide and MHC molecule in silico, thus to predict the potential binding peptide. Although the results of in silico predictions are not so accurate as that of in vitro binding analysis, this method could be used to effectively screen out potential epitopes from a large pool of peptides, thus saving experimental expenditure and time. In contrast, in vitro testing, such as ELISpot assay and cytochrome staining, is more precise. But such methods are depicted as time-consuming, complicated and required multiple determinations to improve accuracy.

Combined with the advantages of various T cell epitope discovery strategies, Creative Biolabs' excellent researchers has developed an advanced technology platform for epitope discovery based on the tetramers screening and ELISpot assay, which could meet our customers’ various demands.

The platform provides highly sensitive and reproducible cell assay service, including high throughput MHC class I, MHC class II binding peptides assays, antigen-specific CD4+ and CD8+ T cell immune responses assays. For each customer's project needs, our team will optimize the experimental process in order to achieve the best experimental results and save your money and time.

Featured Applications of Our CreMap™ T Cell Epitope Discovery Service

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