| Overview | |
|---|---|
| Description | Creative Biolabs offers a wide range of reporter bioassays kits to characterize and develop novel monoclonal antibody (mAb) and cell-based therapeutics. The PD-1+TIGIT Combination Bioassay Kit is a bioluminescent cell-based assay that can be used to measure the potency and stability of antibodies and other biologics targeting PD-1 and TIGIT. |
| Features | Convenience and minimizes variability; Easily applicable to high-throughput workflows. |
| Applications | To measure the potency and stability of antibodies and other biologics targeting PD-1 and TIGIT. |
| Application Notes | It is recommended to conduct a preliminary experiment before the formal experiment. |
| Molecular Mechanism of Action | The Bioassay Kit consists of two genetically engineered cell lines, PD-1+TIGIT Effector Cells and PD-L1+CD155 aAPC/CHO-K1 Cells. When co-cultured, PD-1 inhibits TCR pathway-activated luminescence, and TIGIT inhibits CD226 pathway-activated luminescence. The addition of anti-PD-1 Ab blocks PD-1 binding to PD-L1, resulting in full TCR pathway activation. The addition of anti-TIGIT Ab blocks the TIGIT/CD155 interaction, thereby re-establishing CD226 pathway-activated luminescence. Blocking of PD-1/PD-L1 and TIGIT/CD155 can be detected in a dose-dependent manner by addition of Bio-Glo Reagent and quantitation with a luminometer. |
| Qualified With | Prequalified according to ICH guidelines |
| Assay Measures | Uses Bio-Glo Luciferase Assay System |
| Research Areas | Drug discovery; Function assay |
| Specification | |
|---|---|
| Size | 1EA |
| Components | 1 vial x5 PD-1+TIGIT Effector Cells (0.5ml x5) 1 vial x5 PD-L1+CD155 aAPC/CHO-K1 Cells (0.5ml x5) 36ml x5 RPMI 1640 Medium 25ml x5 Ham's F-12 Medium 4ml x5 Fetal Bovine Serum 1 vial x5 Bio-Glo Luciferase Assay Substrate (lyophilized) 10ml x5 Bio-Glo Luciferase Assay Buffer |
| Target | PD-1&TIGIT |
| Species Reactivity | Human |
| Target/Description | PD-1 and TIGIT are both immune inhibitory receptors that are expressed on T cells. Engagement of PD-1 by PD-L1 on an adjacent cell inhibits TCR signaling and TCR-mediated proliferation, transcriptional activation and cytokine production. The ligand for TIGIT is CD155. TIGIT negatively regulates NK cell killing and T cell activation by competing with CD226 for binding to CD155 and by directly interfering with CD226 homodimerization. |
| Alternative Names | PDCD1; VSTM3 |
| UniProt ID | Q15116; Q495A1 |
| Compatibility | Each kit contains sufficient reagents for 600 assays using the inner 60 wells of ten 96-well plates which can do 20 antibody analysis (10 concentration dose*3 repeats). |
| Format | 96- and 384-well plate formats |
| Storage | Upon arrival, immediately transfer the cell vials to below -140°C (freezer or liquid nitrogen vapor phase) for long-term storage. Do not store cell vials submerged in liquid nitrogen. Do not store cell vials at -80°C as this will negatively affect cell viability and performance. Store Bio-Glo Luciferase Assay Buffer, Bio-Glo Luciferase Assay Substrate and Fetal Bovine Serum at -20°C. Avoid multiple freeze-thaw cycles of the serum. We recommend preparing Bio-Glo Reagent immediately before use. Do not store and use the reconstituted reagent. Store RPMI 1640 Medium at 4°C protected from fluorescent light. |
| Handling | Please place the product at proper storage condition once received. |
| Shipping | Components are shipped separately by dry ice and wet ice. |
For Research Use Only.
