PathSpecific™ DNMT3L Methyltransferase Assay Kit (ELISA) (CAT#: ZL-0124-WR51)

Overview
DescriptionThe kit is an advanced tool used for accurately quantifying levels of Mouse DNA[cytosine-5]-methyltransferase 3-like in various biological samples, including tissue homogenates, cell lysates, and other biological fluids. This kit is highly applicable for research purposes, allowing for precise measurements of DNMT3B activity in screening and profiling applications.
FeaturesReady-used for enzymatic activity assay.
ApplicationsKit is used for compound screening and profiling.
Application NotesOur ELISA kit is best suited for detecting native samples instead of recombinant proteins.
Research AreasKinase Drug Discovery; Signal Pathway;
Specification
Size96 tests
ComponentsThe kit is supplied with an assay kit including Pre-coated 96-Well Microplate, Standard, Standard Diluent Buffer, Wash Buffer, Detection Reagent A & B, Diluent A & B, TMB Substrate, Stop Solution, and Plate Sealer.
TargetDNMT3L
Species ReactivityMouse
Target/DescriptionThis ELISA kit uses a sandwich assay to detect DNMT3L. After coating the plate with an antibody, samples and biotin-conjugated reagent are added. After washing, an HRP-conjugated reagent is added and unbound conjugates are removed. TMB substrate is used to measure the HRP enzymatic reaction, with the resulting color change being directly proportional to the amount of DNMT3L present. The OD at 450 nm is measured to determine the concentration of DNMT3L.
Sensitivity0.1 ng/ml
FormatSupplied with an ELISA assay kit
StorageStore at 4°C
HandlingPlease store the kit according to the storage instructions in the kit's manual upon receipt.
ShippingIce packs
Regulatory StatusRUO

For Research Use Only.

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