| Overview | |
|---|---|
| Description | This kit is a fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP10 (Ubiquitin Specific Peptidase 10) for screening and profiling applications. |
| Features | Ready-used for deubiquitination assay |
| Applications | Useful for examing enzyme kinetics and screening small molecule inhibitors for drug discovery in high throughput screening (HTS) applications. |
| Assay Measures | Fluorogenic |
| Research Areas | Kinase Drug Discovery; Signal Pathway |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | This kit includes enough purified recombinant human USP10 protein, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions. |
| Target | USP10 |
| Target/Description | Analyzing the impact of an inhibitor on USP10 activity involves preincubating the enzyme with or without the inhibitor before introducing the Ub-AMC substrate. This assay's validation included the utilization of UbAldehyde, a potent inhibitor that targets various DUB subfamilies including Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases. |
| Format | Fluorometric assay kit. |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please handle the product at appropriate storage condition once received. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
