Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications. Highlights
Creative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsTo overcome the disadvantage of trifunctional antibody production as a mixture, scientists from Creative Biolabs has introduced knobs-into-holes amino acid changes to perform rational design in antibody engineering. The principle is that heavy chains (H) of human immunoglobulin IgG interact at the level of their CH3 domains directly, whereas, at the level of their CH2 domains, they interact via the carbohydrate attached to the Asn N84.4 in the DE turn. Therefore, the interface between CH3 domains can play a key role for the efficient formation of heterodimers.
Fig. 1 Trifunctional antibody enforced through knobs-into-holes mutations in CH3
domains. (Rouet and Christ 2014)
The figure above presents the obtained products from the knobs-into-holes engineering for trifunctional antibody production. Comparing to quadromas, this method avoids the impurity problem of produced tri functional antibody.
Heterodimerization of antibody heavy chains specific for different antigens can be enforced through knobs-into-holes mutations in CH3 domains; as a result, correctly assembled bispecific antibodies can be expressed either in E.coli or mammalian cells [e.g. human embryonic kidney (HEK) cells] co-transfected with constructs encoding all four chains.
Moreover, it is very possible that the modification on Fab region to introduce the knobs-into-holes area may affect the specificity and binding affinity of the antibodies. Usually, a library of antibody chain with engineered mutations will be constructed. And directed protein evolution methods such as phage display will be applied to select the stable and functional antibody chain to construct the tri functional antibody.
Scientists in Creative Biolabs have successfully established the method to produce custom bispecific antibody according to client's requirement. If interested, please contact us.
Reference
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
