Custom X-Ray Crystallography Services

Creative Biolabs provides the most cost-effective X-ray Crystallography services for determining three-dimensional structures of proteins and other macromolecules. Equipped with state-of-the-art X-ray diffraction data collection equipments and structure analysis software, we specialize in the co-crystallization of target proteins with small molecule compounds.  Our unsurpassed capacity in the field allows a full range of services including gene expression, protein purification, macromolecular crystallization, X-ray characterization of crystals, processing and quality analysis of data, structure determination, molecular modeling, molecular docking and structure visualization and analysis. Stage-wise services such as crystallization screening, in-house X-ray diffraction data collection and structure determination are also available upon request.

Gene Cloning and Protein Expression
Creative Biolabs is equipped with state-of-the-art fermentation systems for production of recombinant protein in E. coli, yeast, baculovirus-insect, and mammalian cell systems.  We will work with you to determine the best protocol for your target protein. Our laboratory is equipped with both traditional chromatography systems and HPLC for large-scale protein purification. We test all purified proteins for purity, aggregation state and activity using enzyme assays, dynamic light scattering, UV spectrometry, mass spectrometry and amino acid sequencing.

Crystallization Screening
Creative Biolabs can test the crystallization conditions for freshly purified target proteins and compounds provided by clients. We'll need approximately 10 mg of purified, concentrated protein for initial trial using a general screen. We search for both conditions that generate co-crystals (protein plus ligand) and those produce apo-crystals for socking with ligand. If crystals are obtained, we will fine-tune the conditions to generate diffraction-grade crystals. We will send you pictures of the crystals as well as conditions for generating them.

X-ray Diffraction Data Collection
Creative Biolabs collect X-ray diffraction data using our powerful in-house X-ray generator and send the data collected to our customers.

Structure Determination
Creative Biolabs has extensive experience in structure determination and can provide model building and refinement services based on X-ray diffraction data collected. If the target protein has homologues with known 3-D structure in the PDB, our crystallographers can use molecular replacement method to build the model. Importantly we can also solve de novo structures using multi-wavelength X-ray methods with data collected using synchrotron sources. As evidenced by quite a few publications by our staff scientists in top journals like Nature and Science, we are professional in resolving the structure of membrane proteins and other difficult proteins.

High-resolution protein structure provides a solid foundation for the development of target specific drugs. Our flexible custom crystallography services make your drug discovery goals in schedule.