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Peptide Immunogen Phage Display Platform

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Peptide immunogen phage display platform was developed to efficiently produce monoclonal antibodies against peptide immunogens. This platform is based on our unparalleled expertise in phage display technology, in which the cDNA encoding a peptide is fused with the pVIII gene of a filamentous M13 phage. The peptide is expressed and displayed on the surface of filamentous phage at over 200 copies per phage particle. The displayed peptide together with the carrier phage virions is used for animal immunization; here the phage virion serves as one of the best immunization adjuvants. This platform supports antibody production in mice, rats, rabbits, chicken, sheep and llama. For monoclonal antibody production, antibody clones that are specific for the peptide but not the phage particles are then selected for further characterization.

Compared with conventional peptide-conjugation based methods, this platform supports the formation of close-to-nature structure of the peptide that is bacterially expressed, processed and displayed on phage surface, thus increasing the chance to raise antibodies that can recognize the original antigen, from which the peptide immunogen is derived. Also, there are over 200 copies of the peptide on each phage virion, while a peptide conjugate has fewer copies of the peptide on each molecule. Importantly, the carrier phage is much stronger in raising immune response in comparison with carrier protein OVA, KLH and BSA. Furthermore, peptide conjugation usually alters the structure of the peptide, while our method does not.

If used in conjunction with our unique Magic Mouse Adjuvant, this platform allows generation of hundreds of mouse hybridoma clones against a peptide immunogen with one hybridoma fusion, thus providing a large pool of candidate antibodies for further selection of diagnostic or therapeutic antibody leads.

This platform is also the most cost-effective technology in discovering peptide vaccines, a type of subunit vaccines in which a peptide of the original pathogen is used to immunize an organism. The key challenge in developing an excellent peptide vaccine is to determine the most efficient peptides from the protein sequences of the pathogen. Our peptide immunogen phage display platform allows determination of the most promising vaccine peptides by phage-displaying a peptide library and then identifying the peptides that recognized by anti-sera.

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