Premade Phage Display Peptide Libraries
 

Besides using the publically available peptide libraries, we also have 3 peptide libraries that were constructed in house.

TriCo-16^TM Phage Display Peptide Library is a pIII-fusion16-mer random peptide library based on a true phage vector. The random peptides are encoded by DNA sequences that were randomized using Trinucleotide Phosphoramidites (Trimer Codons) of bacterial preference, thus completely avoiding stop and non-sense codons. In addition, the trimer codon for cysteine was excluded. The current size of this library is 2.60×10¹⁰.

TriCo-20^TM Phage Display Peptide Library is a pIII-fusion20-mer random peptide library based on a true phage vector. The random peptides are encoded by DNA sequences that were randomized using Trinucleotide Phosphoramidites (Trimer Codons) of bacterial preference, thus completely avoiding stop and non-sense codons. In addition, the trimer codon for cysteine was excluded. The current size of this library is 1.70×10⁹.

As validated by quality control DNA sequencing, more than 97% of randomly picked clones from these two libraries contained dsDNA inserts, and only an insignificant percentage of them harbored longer or shorter diversified peptides. None stop codons were founded as expected. Also, there was no single nucleotide deletion or insertion detected; importantly, non-sense frame-shift mutation was completely absent, demonstrating the beauty of trimer-codon strategy. Moreover, no Cys encoding codon was identified in the peptide library as designed. These Random Peptide Libraries are the best random 16-mer and 20-mer peptide libraries in terms of their quality and diversity.

Phage Display Peptide-10^TM Library is a pIII-fusion 10-mer peptide library constructed in a phagemid vector using NNK method with a size of 1.2×109.