Construction of Phage Display Libraries

We are professional in construction of various phage display libraries, including cDNA libraries, peptide libraries and scFv/Fab antibody libraries. We normally generate libraries with a diversity of n*10⁸; we are able to generate libraries of n*10^9-10. In case you need a special cDNA, peptide or antibody library, you can rely on our extensive experience. For peptide libraries with inserts longer than 8-mer [and up to 30-mer], we usually use one of three nucleotide doping strategies [NNK, Split-mix-split synthesis or Tri-mer codon] to maximize the amino acid sequences encoded by the inserted DNA sequences. We can make the both pIII-fusion libraries and pVIII-fusion libraries. For scFv/Fab library construction, we can use both human plasma cells and splenocytes from naïve or immunized mice. We are also professional in producing fully or semi-synthetic antibody libraries.

     o Library types:
          • Phage display peptide libraries;
          • Phage display antibody libraries, including immunized antibody libraries, naïve antibody libraries and
            semi-synthetic/synthetic antibody libraries.
          • cDNA phage display libraries.

     o Construction vectors:
          • New England BioLab's true phage vector, M13KE, for construction of peptide library;
          • pIII fusion phagemid for construction of peptide library;
          • Novagen's T7Select Phage Display vector for construction of cDNA library.

     o Diversity of synthetic libraries:
          • "Hard randomization": complete diversity is introduced by using degenerate codons encoding all
            twenty natural amino acids
          • "Tailored randomization": incomplete or biased diversity is introduced by using degenerate codons 
            encoding a subset of natural amino acids
          • Diversification: degenerate codons are synthesized using trimer phosphoramidites corresponding 
            to 20 natural amino acids, which exclude stop codons.

     o Library capacity:
          • Large phage display libraries containing 10^7-10 variants