Screening of Phage Display Libraries

Our staff scientists have extensive experience in screening of phage display peptide, cDNA and scFv/Fab libraries. In particular, by conducting library screening, panning, for 4 cycles, we normally get scFv/Fab antibodies of an affinity of 10^-7. By constructing serial sub-libraries of the isolated scFv/Fab antibodies, our proprietary protocol allows increase of the affinity of the scFV antibodies from 10^-8 to 10^-9. We have successfully obtained a scFV antibody that has an extremely high affinity of 10^-12, whose binding to the antigen is essentially irreversible.

     o Phage Display Libraries:
          • Libraries commercially available
          • In house premade libraries (HuScL®, HuSdL®, HuFabL®, MuScL®)
          • Custom constructed library or libraries from the customer

     o Targets:
          • Purified antigens;
          • Un-purified antigens;
          • Intact cells, tissues or any in vivo selection systems required by the customer.

     o Applications:
          • Identify enzyme inhibitors
          • Map epitopes on antigens;
          • Select new antibodies;
          • Discover/validate new therapeutic targets;
          • Discover interaction between ligand and receptor;
          • Select any peptide/protein binders;