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ScFv/Fab productionBesides the full range of phage display antibody library construction and screening services, we are also recognized by our downstream services in antibody engineering and antibody biomanufacturing. We have built up our expertise in antibody engineering based around monoclonal antibodies derived from both hybridoma technology and phage display antibody library technology. Creative Biolabs has established a solid platform for scFv and Fab production. There are three routes to have a scFv or Fab targeting a specific antigen. The first is to generate a mouse hybridoma clone, then convert full IgG [or IgM] into a scFv or Fab; the second approach is to create an immunized phage display scFv or Fab mouse library, then use the antigen to screen the library; the third method is to use the antigen to screen a premade scFv or Fab antibody phage display library [usually a human one] to get scFv or Fab clones directly. We are able to convert chicken IgY into scFv or Fab fragments. We have a series of well-tested bacterial expression systems established in house, which allow high level expression of human and mouse scFv and Fab antibodies in a highly soluble form with binding activity.
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