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Premade Phage Display Peptide Libraries

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Besides using the publically available peptide libraries, Creative Biolabs also provide various peptide libraries that were constructed in house:

We carry two good linear peptide libraries: one is 16-mer and the other is 20-mer. They are based on the same true phage system as NEB Ph.D. peptide libraries. In theory, there are about 3-5 copies of peptide on each phage virion.

TriCo-16™ Phage Display Peptide Library
  1. Type: pIII-fusion, True Phage Display
  2. Format: 16-mer peptide library
  3. Capacity: 2.60×1010
  4. Trimer codons technology
  5. Avoid stop and non-sense codon
  6. Trimer codon for cysteine was excluded

TriCo-20™ Phage Display Peptide Library
  1. Type: pIII-fusion, True Phage Display
  2. Format: 20-mer peptide library
  3. Capacity: 1.70×109
  4. Trimer codons technology
  5. Avoid stop and non-sense codon
  6. Trimer codon for cysteine was excluded

As validated by quality control DNA sequencing, more than 97% of randomly picked clones from these two libraries contained dsDNA inserts, and only an insignificant percentage of them harbored longer or shorter diversified peptides. None stop codons were founded as expected. Also, there was no single nucleotide deletion or insertion detected; importantly, non-sense frame-shift mutation was completely absent, demonstrating the beauty of trimer-codon strategy. Moreover, no Cys encoding codon was identified in the peptide library as designed. These Random Peptide Libraries are the best random 16-mer and 20-mer peptide libraries in terms of their quality and diversity.

Creative Biolabs also provide the following specific peptide libraries:

F88-4 Library
1. Type: pVIII-fusion (~150 copies)
2. Format: 15-mer peptide library
3. Capacity: 2.0×109

F88-Cys1 Library
1. Type: pVIII-fusion (~150 copies)
2. Format: 10-mer peptide library
3. Capacity: 5.6×108

F88-Cys2 Library
1. Type: pVIII-fusion (~150 copies)
2. Format: 11-mer peptide library
3. Capacity: 2.8×109

F88-Cys3 Library
1. Type: pVIII-fusion (~150 copies)
2. Format: 12-mer peptide library
3. Capacity: 5.5×107

F88-Cys4 Library
1. Type: pVIII-fusion (~150 copies)
2. Format: 12-mer peptide library
3. Capacity: 5.5×107

F88-Cys5 Library
1. Type: pVIII-fusion (~150 copies)
2. Format: 12-mer peptide library
3. Capacity: 5.9×108

F88-Cys6 Library
1. Type: pVIII-fusion (~150 copies)
2. Format: 14-mer peptide library
3. Capacity: 2.7×108

FUSE5-6 Library
1. Type: pIII-fusion
2. Format: 6-mer peptide library
3. Capacity: 2.0×108

FUSE5-15 Library
1. Type: pIII-fusion
2. Format: 15-mer peptide library
3. Capacity: 2.5×108

Monobody-1 Library
1. Type: pIII-fusion, Monobody
2. Capacity: 2.20×1010
3. Phage Display
4. Trimer codons technology

Monobody-2 Library
1. Type: pIII-fusion, Monobody
2. Capacity: 2.80×1010
3. Phage Display

Peptide-10™ Library
1. Type: pIII-fusion, Phagemid Phage Display
2. Format: 10-mer peptide library
3. Capacity: 1.2×109
4. NNK method

Protease-10A Library
1. Format: HiAffi tagged 10-mer peptide library
2. Capacity: 1.1×1010
3. Phage Display
4. C-terminal is labelled by protein A for purification

Protein A Domain Library
1. Type: pIII-fusion, Protein A [C Domain]
2. Capacity: 1.60×1010
3. Phage Display

Protein G Domain Library
1. Type: pIII-fusion, Protein G [C Domain]
2. Capacity: 2.40×1010
3. Phage Display

TriCo-9 Library
1. Type: pIII-fusion, Phagemid Phage Display
2. Format: 9-mer peptide library
3. Capacity: 8.60×1010

TriCo-C9 Library
1. Type: pIII-fusion, Phagemid Phage Display
2. Format: Cyclic 9-mer peptide library
3. Capacity: 8.60×1010

Vrisko Peptide Library
1. Type: pVIII-fusion
2. Format: 8-20-mer peptide library
3. Capacity: 5.0×109
4. Includes both linear (X8) and disulfide-constrained peptides
5. Provides pre-organized potential binding motifs
6. Composed of 15 different scaffolds (including X4CX2GPX4CX4, X5CX8CX5, X5CX9CX4 and X4CX9CX5)
7. Features different arrangements of conformation biasing disulfides and randomized residues




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