Custom Full-Length Gene Synthesis

Custom gene synthesis is a solid component of the all-inclusive protein expression services offered by Creative Biolabs. Gene synthesis is a technology that produces double-stranded DNA molecules with specified sequences from dNTPs. Nowadays, many genes are physically available from commercial suppliers. However, there are a significant number of genes that exist only as digital sequences. Also, Biological and Pharmaceutical research require genes of man-made and predicted sequences that do not exist naturally. Custom gene design and assembly provide a unique approach to obtain these otherwise unavailable genes.

     o Synthesis of genes with all possible sequences from 50bp to 10kb;

     o Comprehensive gene design FOR FREE;
          • Codon optimization
          • RNA secondary structure optimization
          • Restriction enzyme sites inclusion
          • Addition of N- or C-terminal affinity tag
          • Integration of upstream ribosome binding sites or a Kozak consensus sequences for optimal expression
          • Introns removal
          • Addition of elimination of protein modification sites such as phosphorylation, glycosylation or Pegylation sites
          • Division of genes into modules or cassettes for protein domain swapping, insertion, deletion or duplication
          • Introduction of specific mutations within a particular gene module
          • Chimeric/fusion protein production

     o Extensive manufacturing experience with over 30,000 genes synthesized;

     o Downstream gene cloning and protein expression services immediately available;

     o The lowest price.

Creative Biolabs provides gene synthesis with the lowest price and highest quality guaranteed. In particular, we offer the entire expertise-demanding gene design service for free. Also, the sequences of synthesized genes are finally confirmed by bidirectional DNA sequencing. Our clients need confirm the sequence we design before we initiate DNA synthesis.

Deliverables:
          • Lyophilized plasmid DNA containing the synthesized gene. Our standard vectors are pUC18/19 and pBluescript vectors.
            Other vectors are available at additional cost;
          • Final DNA sequencing report;
          • Gene synthesis report.