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Solid-phase Library Screening

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Creative Biolabs is a well-recognized service provider of phage display technology. We have developed a serial of advanced phage display library screening methods for selecting antibodies with high affinity and specificity. Solid-phase screening is a valuable method that is rapid, efficient and relatively inexpensive for selecting the binding antibodies, identifying epitopes, etc. We offer an optimized solid-phase screening strategy which guarantees the success of finding binders targeting various types of antigens.

Phage display is one of the most effective technology for antibody generation since it links the phage phenotype and its encapsulated genotype. In general, the peptide or antibody library is constructed by fusing interested proteins with pIII, pVIII or other capsid proteins and displaying on the surface of the M13 phage particle. Our seasoned scientists are confident in generating high-quality libraries with diversity over 1010.

Phage display library has become a popular tool for treating autoimmune and cancer diseases. While the biopanning stage plays a crucial role in the enrichment and isolation of desired binders that can effectively recognize the interested targets. This stage involves a repeated cycles of incubation, washing, elution and amplification. Multiple rounds of selection cycle are required for obtaining monoclonal phage antibodies with desired binding affinity. Enzyme-linked immunosorbent assay (ELISA) could also be incorporated to determine the activity after the selection. As the most widely used biopanning method, solid-phase screening indicates an effective selection approach that can apply to most of binder selection projects. A series of solid-phase, like microtiter plate wells, PVDF membranes, could be used for target molecule immobilization.

Figure 1. Process of phage display library selection.

Several components in the screening system affect the selection result, such as the type of solid support, time of binding, washing and elution, and antigen concentration. We have developed an optimized procedure which minimizes the possibility of selecting target-unrelated binders. In order to reduce the binding to plastic, several methods could be applied, such as blocking the surface or immobilizing the target using an alternative approach other than direct adsorption. The density of the target could be increased to reduce the possibility of selecting background binders. Also, subtractive selection could be used to remove background binders.

Creative Biolabs is highly experienced in phage display technology and has proven records of generating high-affinity antibodies in a cost-effective and timely manner. Our solid-phase screening is optimized and precisely controlled, therefore avoiding the possibility of false-positives. Combined with other advanced technologies, we could offer the most comprehensive services and products based on our phage display platform, from immunization, library construction and screening to antibody engineering. Our scientists are pleased to offer tailored solutions to meet our customers’ different projects.

Reference:
Smolarek D, Bertrand O, Czerwinski M. Variable fragments of heavy chain antibodies (VHHs): a new magic bullet molecule of medicine? Zmienne fragmenty przeciwciał ciężkołańcuchowych (VHH): nowa magiczna kula medycyny?[J]. Journal cover, 2014, 68.




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