Adipose-on-a-chip Model for Drug Screening

Adipose-on-a-chip model is an experimental platform in metabolic diseases to mimic adipose dysfunction. Our new technologies relating to the isolation, characterization, and manipulation of primary human adipose-derived cells and scaffolds have advanced the adipose biology field considerably. Creative Biolabs can offer you with up-to-date adipose-on-a-chip models to screen potential drugs accurately.

Creative Biolabs' Adipose-on-a-chip Model

The generation of our adipose-on-a-chip model presents a compartmentalized microfluidic co-culture system that gives a constant rate of nutrient supply as well as waste removal, resembling the microvascular networks of the in vivo environment, which also mimics adipose tissue hypertrophy and inflammation via excessive free fatty acid (FFA) loading.

Fig.1 Adipose-on-a-chip.¹

The adipose chip model supports the 3D perfusion culture of human differentiated adipocytes with improved adipogenic phenotypes as compared to conventional 2D well-plate cultures. We developed an induction protocol to mimic exposure to high dietary lipids using two different combinations of FFA that were conjugated to Bovine Serum Albumin, similar to how plasma FFA is bounded to serum albumin in vivo. Adipocytes treated with palmitate and oleate (PA-OA) maintained their diseased phenotype for at least 3 days upon withdrawal of PA-OA. Finally, we applied the PA-OA disease induction protocol to our adipose chip model.

Endpoints services

• Histological Staining and Evaluation
• Metabolic Activity Assessment
• Secretome Profiling
• Cell Viability Staining Analysis
• RNA and Protein Expression Analysis
• Cytokine Profiling
• Microscopy Techniques:
  • Bright-field Imaging
  • Fluorescent Imaging
  • Confocal Microscopy
  • High-Throughput Screening

Related Services

Along with our diverse model range, we present bespoke supporting assays and preclinical research services listed below to fulfill the research demands of clients across the world. Our team of seasoned scientists, armed with extensive expertise in 3D biology, stand ready to align solutions with your specific requirements. Whenever you require any form of professional technical support, we invite you to contact us to gain deeper insights into our highly customizable 3D models and the supporting services.

Types of Adipose-on-a-chip Model

Table.1 Two types of Adipose-on-a-chip Model at Creative Biolabs.

Applications of Adipose-on-a-chip Model

• Potentially be useful to study the downstream effects of adipocytes on target metabolic tissues such as skeletal muscle and liver in the context of obesity and metabolic diseases.
• Supporting a dynamic 3D culture of adipocytes with enhanced adipogenic phenotype.
• Driving adipocyte inflammation and formation of hypertrophic lipid droplets with free fatty acids of our AOC Model.
• Coupling to liver/muscle-on-chip platforms to study the effects of adipose tissue on other metabolic organs, with linkage to other organoids to examine the impact of biological aging on metabolic function.

Our Advantages

Creative Biolabs' adipose-on-a-chip models push studies on how fat functions in the body, leading to new interventions for related diseases, including metabolic diseases, diabetes, heart disease, arthritis, and other serious health conditions, which will accelerate and enhance the drug discovery pipeline for obesity, diabetes, and metabolic diseases by reducing the preclinical evaluation process and improving predictive accuracy. By applying our adipose chip in your study, you can surely recreate a closed-loop recirculatory platform by using modular microfluidic platforms which are capable of incorporating multiple organ-on-chips systems along with a micropump.

In addition to adipose-on-a-chip model, we have varieties of choices, talk to our experts to address your needs:

Related Sections:

• Single Organ-on-a-Chip Model Introduction
• Multiple Organs-on-a-Chip Model Introduction
• Barrier Tissue On-a-Chip Model Introduction
• Customized Tissue-on-a-Chip Model Introduction
• Disease-on-a-Chip Model Introduction