Gastrointestinal Toxicity Evaluation by 3D Cell Model

Gastrointestinal toxicity evaluation with 3D cell models focuses on applying in vitro cellular techniques to examine the effects of the tested material on the cells in the models, with a view to understanding the toxic effects, dose-dependent exposure characteristics, and the toxic response mechanism of the tested material. Creative Biolabs provides gastrointestinal toxicity evaluation services by 3D cell models to help clients recognize the potentially toxic effects of the tested materials.

3D Cell Modeling

Cell type
Apply suitable cell types relevant to the gastrointestinal tract, such as gastric epithelial cells (e.g., human gastric adenocarcinoma AGS cell line) or intestinal cells (e.g., human colon cancer Caco-2 cell line).
3D cell culture
Suspending selected cell seeds in the biological matrix induces cells to form 3D structures.
Co-culture of various cell types
To assess intestinal absorption and gastrointestinal toxicity, gastric epithelial cells and intestinal cells are combined in one model to emulate the entire gastrointestinal tract.

Evaluation Approaches

Cell viability assay	Monitoring of cell viability by staining or real-time detection methods such as fluorescent reactive dyes or cytochrome enzyme activity.
Cell morphology and structure assessment	Observing the morphology and structure of cells in 3D models with microscopy, to detect the cell tight connection and the stability of the tissue structure.
Functional assays	Detection of the functional performance of cells in the 3D model, such as cell differentiation status, cell membrane permeability, expression of specific proteins, etc.
Cytotoxicity evaluation	Evaluating toxicity by measuring cell metabolic activity, apoptosis and necrosis levels, DNA damage, and other indexes.
Dose-related exposure signatures	Setting up multiple combinations of test conditions to obtain toxic response signatures such as exposure time, exposure frequency, and exposure dose.

Schematic diagram of the 3D intestinal cell model. Fig 1. Establishment of the 3D intestinal cell model.¹

Strength of 3D Cell Model-based Evaluation

2D cell model	3D cell model
Risk of losing original tissue structures and behaviors. Presence of altered metabolism, phenotype, and gene expression. Impact on cell model growth and response to tested agents.	Meshwork structure for cell growth constituted with matrix proteins and skeleton facilitates the induction of cell polarity. Cellular functions and complex inter-cellular interactions involve reliance on the matrix, consistent with the in vivo condition. A more accurate representation of cell biological actions in response to toxic stimuli.

More Gastrointestinal Toxicity Evaluation Services

Gastrointestinal Toxicity Evaluation by Intestinal Organoid Model

Creative Biolabs builds 3D cell models for the evaluation of gastrointestinal toxicity, with lucid protocols and multiple endpoint assays reflecting more realistic and reliable predictive results. Please contact us to accelerate your assay.

Reference

Fu, Linglin et al. "Establishment of a 3-Dimensional Intestinal Cell Model to Simulate the Intestinal Mucosal Immune System for Food Allergy Investigations." Frontiers in immunology. 13 (2022): 853443. Distribution under Open Access license CC BY 4.0, without modification.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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