Should I aliquot the samples for long-term storage?

Yes, please aliquot the samples to minimize freeze/thaw cycles.

Do the lysates contain dissociating agents?

No dissociating agents were added during preparation, but if needed, they can be incorporated prior to heating.

HT-1080 Cell Nuclear Lysate (CAT#: DBFF-0825-HX815)

Datasheet

Overview

HT-1080 Cell Nuclear Lysate from Creative Biolabs is provided at a defined protein concentration and is suitable for a wide range of protein-related applications. The cell lysate is prepared by washing cells with PBS and lysing them on ice in a modified RIPA buffer supplemented with a broad-spectrum protease inhibitor cocktail and phosphatase inhibitors to preserve protein integrity.

Quantity:

* For Research Use Only. Not For Clinical Use.

Specifications

Size

0.2 mg

Concentration

4 mg/ml

Form

Liquid, sterile filtered

Species

Human

Cell Line

HT-1080

Lysate Type

Cell nuclear lysate

Product details

Protein Assay Method

Modified Lowry assay

Stimulation Factor

Unstimulated

Induction Concentration

None

Buffer

1X RIPA Buffer with HALT Protease and Phosphatase Inhibitors

Preservative

Preservative-free

Applications

Western blot, immunoprecipitation, capture ELISA or others.

Handling Notes

For Western blot, dilute lysates to the desired concentration in SDS-PAGE buffer with a reducing agent (2-mercaptoethanol or DTT) and heat at 95°C for 5 minutes before use.

Storage and shipping

Shipping Condition

Dry ice.

Storage

Store at -80°C or below. It is recommended to aliquot the lysate and avoid repeated freeze-thaw cycles.

Shelf Life

3 months from the date of receipt.

FAQs

Should I aliquot the samples for long-term storage?
Yes, please aliquot the samples to minimize freeze/thaw cycles.
Do the lysates contain dissociating agents?
No dissociating agents were added during preparation, but if needed, they can be incorporated prior to heating.

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For Research Use Only | Not For Clinical Use