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Human Primary Pulmonary Alveolar Epithelial Cell Lysate (CAT#: DBFF-0825-HX920)

Datasheet

Overview

Human Primary Pulmonary Alveolar Epithelial Cell Lysate is derived from primary cells at a lower passage using a modified RIPA buffer. After dilution, the lysate is stored in a buffer containing Tris-HCl (pH 6.8), 5% glycerol, 1% SDS, bromphenol blue, and β-mercaptoethanol. This ready-to-use sample offers a time and energy-saving option for biological researchers.

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Quantity:

Specifications

Size
0.2 mg
Concentration
Batch-dependent
Form
Liquid
Species
Human
Lysate Type
Cell lysate

Product details

Protein Assay Method
Bicinchoninic acid (BCA)
Stimulation Factor
Unstimulated
Induction Concentration
None
Buffer
SDS-PAGE loading buffer (Tris-HCl pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue, 67% Beta-mercaptoethanol)
Preservative
Preservative-free
Applications
Western blot
Handling Notes
For optimal results, heat the lysate at 95°C for 5 minutes prior to loading, then immediately place on ice to cool before applying to an SDS-PAGE gel.

Storage and shipping

Shipping Condition
Dry ice.
Storage
Store at -80°C or below. It is recommended to aliquot the lysate and avoid repeated freeze-thaw cycles.

FAQs

  1. How is the lysate prepared?

    The lysate is prepared from early passage cells using a modified RIPA buffer. The concentration is determined before dilution and storage in a buffer.

  2. How many freeze/thaw cycles can the lysate withstand?

    We have not verified the number of freeze/thaw cycles the lysate can withstand, but we recommend aliquoting the sample for storage to minimize repeated freeze/thaw cycles.

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For Research Use Only | Not For Clinical Use

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