Should I aliquot the samples for long-term storage?

Yes, please aliquot the samples to minimize freeze/thaw cycles.

Do the lysates contain dissociating agents?

No dissociating agents were added during preparation, but if needed, they can be incorporated prior to heating.

MCF-7 Cell Nuclear Lysate (CAT#: DBFF-0825-HX837)

Datasheet

Overview

MCF-7 Cell Nuclear Lysate from Creative Biolabs is provided at a defined protein concentration and is suitable for a wide range of protein-related applications. The cell lysate is prepared by washing cells with PBS and lysing them on ice in a modified RIPA buffer supplemented with a broad-spectrum protease inhibitor cocktail and phosphatase inhibitors to preserve protein integrity.

Quantity:

* For Research Use Only. Not For Clinical Use.

Specifications

Size

0.2 mg

Concentration

1 mg/ml

Form

Liquid, sterile filtered

Species

Human

Cell Line

MCF-7

Lysate Type

Cell nuclear lysate

Product details

Protein Assay Method

Bicinchoninic acid (BCA)

Stimulation Factor

Unstimulated

Induction Concentration

None

Buffer

1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)

Preservative

Preservative-free

Applications

Western blot, SDS-PAGE.

Handling Notes

For Western blot, dilute lysates to the desired concentration in SDS-PAGE buffer with a reducing agent (2-mercaptoethanol or DTT) and heat at 95°C for 5 minutes before use.

Storage and shipping

Shipping Condition

Dry ice.

Storage

Store at -80°C or below. It is recommended to aliquot the lysate and avoid repeated freeze-thaw cycles.

Shelf Life

One year from the date of receipt.

FAQs

Should I aliquot the samples for long-term storage?
Yes, please aliquot the samples to minimize freeze/thaw cycles.
Do the lysates contain dissociating agents?
No dissociating agents were added during preparation, but if needed, they can be incorporated prior to heating.

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For Research Use Only | Not For Clinical Use