Neurotoxicity Evaluation by 3D Microtissue Model

To assist clients in gaining a comprehensive and accurate understanding of the potential neurotoxicity of drugs or chemical substances, Creative Biolabs offers neurotoxicity evaluation services based on 3D microtissue models.

What is a 3D Microtissue Model?

A 3D microtissue model is a culture of different cell types within the same tissue, cultivated together in a 3D space to recreate the microenvironment of the tissue and simulate the complex structure and functionality found in living organisms. These models utilize scaffold-free hanging drop techniques or cell culture within matrices or gels, allowing cells to suspend or embed, thus forming intricate 3D structures freely.

3D Spheroid Model	3D Microtissue Model	Organoid Model
Formed by spontaneous aggregation of the same type of cells, resulting in a 3D spherical structure.	Created by co-cultivating physiologically relevant different cell types, leading to the formation of complex 3D structures.	Generated from stem cells or tissue-specific cells that spontaneously self-organize into 3D structures in vitro, resembling mini-organs.

What is a 3D Neural Microtissue Model?

A 3D neural microtissue model is a specific type of 3D microtissue model designed to mimic the structure and functionality of neural tissue. These models typically consist of various types of neural cells, such as neurons, glial cells, and neural epithelial cells, along with their interactions within a 3D space.

Within the realm of neural microtissue models, the Neurovascular Unit (NVU) model holds particular significance.

The NVU is a complex structure composed of these cells：

Neurons

The fundamental cellular units responsible for transmitting neural signals.

Glial Cells

Cells that provide support and protection to neurons.

Brain Vascular Endothelial Cells

Forming the blood-brain barrier, controlling the passage of substances into and out of the brain

Their tightly coordinated interactions are crucial for maintaining the normal functioning of the nervous system. The construction of the NVU model aims to simulate this intricate neurovascular structure to study the interactions between neural and vascular cells, the functionality of the blood-brain barrier, and the mechanisms underlying neurological disorders.

Schematic diagram of the development of the Neurovascular Unit in healthy and diseased people. Fig.1 Healthy and diseased NVU.¹

Advantages of 3D Neural Microtissue Models:

Closer to the microenvironment of human neural tissue.
More complex cellular network structure.
More realistic cellular behavior.
Suitable for high-throughput experiments on microplates.
Results are more predictive.

3D Neuro Microtissue Model for Neurotoxicity Assessment

Utilizing 3D neuro microtissue models for comprehensive neurotoxicity evaluation provides more accurate and relevant information, aiding researchers in assessing the harmful effects of chemical substances on the nervous system.

Cell Viability Assays

To determine the toxicity level of chemical substances on neural cells.

Calcium Imaging Assays

To reveal the impact of chemical substances on neural cell signaling.

Marker Expression Analysis

Assessing the influence of chemical substances through immunocytochemistry or immunofluorescence staining.

Neuro Network Function Analysis

To understand the degree of interference of chemical substances on neural network functionality.

High-Content Imaging

Rapidly obtaining more precise results.

Other Available Assays

Creative Biolabs possesses a professional team and advanced technologies with the capability to construct high-quality 3D neuro microtissue models. Particularly, the high-content imaging and analysis technology offers detailed image data and quantitative analysis for clients. If you require the neurotoxicity evaluation, please don't hesitate to contact us. We will provide high-level services and valuable assistance and support for drug development and chemical safety assessments. Additionally, Creative Biolabs offers other advanced experimental models to provide valuable support for drug development and research in the field of neuroscience.

Ex Vivo Human Peripheral Sensory Neurons

Brain Organoid

3D Neuronal Spheroids

Reference

Maurissen, Thomas L et al. "Microphysiological Neurovascular Barriers to Model the Inner Retinal Microvasculature." Journal of personalized medicine. 12,2 (2022): 148. Distribution under Open Access license CC BY 4.0, without modification.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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Research Models

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