Ocular Irritation Evaluation by Human 3D Cornea Epithelial Model

Creative Biolabs offers ocular irritation evaluation services by human 3D cornea epithelial model to assist our worldwide customers in determining the potential of ocular irritation in tested agents.

Human 3D Cornea Epithelial Model Properties

Highly similar in structure and function to human corneal epithelial tissue, showing a multilayered epithelial structure without stratum corneum.
Exhibiting desmosome and hemidesmosome structures similar to those of the human corneal epithelium.
Properly expression of corneal epithelial-specific keratins.
Can be used for eye irritation and toxicity testing of compounds / formulations.
Can be used for efficacy testing of eye care products.

Creation of human corneal epithelial organoids from tissue samples of the human cornea. Fig 1. Establishment of the human corneal epithelial organoid from human cornea tissues.¹

Evaluation Procedures

Materials collection

Normal human corneal epithelial cells from a single donor as seed cells.

Culture

Based on the air-liquid interface culture approach, culturing in serum-free DMEM medium on polycarbonate microporous membrane culture inserts with appropriate pro-epidermal growth factors and pro-differentiation hormones.

Quality control

Visual inspection for no significant defects, no pathogen contamination, sterility, and MTT assay to determine the time for 0.3% Triton X-100 treatment causing half of the reduction in tissue viability meets the standard.

Exposure to the tested agent

Exposure to a given amount of tested agent for a given time (n=2).
Negative control: diH₂O.
Positive control: Methyl acetate.
Test preparation: diH₂O + the tested agent.

Acquisition and processing of experimental data

Commonly evaluated indicators include but are not limited to, cell viability and survival, cell membrane integrity, and cell proliferation and apoptosis, which contribute to a comprehensive evaluation of the irritability of the agent to the model.

Deliverables

Raw data
Mean relative tissue viability
Predicted classification of test material

Mean relative tissue viability ± SD	Classification
>60%	Unclassified, non-irritating
≤60%	Potentially risky, category 1/2, irritating

Experimentation with drug efficacy in Dry Eye Disease (DED) organoid models. Fig 2. Drug testing in Dry Eye Disease organoids.¹

Human 3D Cornea Epithelial Model Advantages

2D cornea epithelial model

Only superfcial information is provided, lacking structural characterization.
Simply modeling approaches do not accurately simulate in vivo test conditions.
The differences that exist between individuals cannot be adequately taken into account.

3D cornea epithelial model

More detailed 3D structural and morphological information, showing features such as comeal geometry and thickness distribution.
The standardized modeling approach allows reproducible simulation of stimulus-model interactions.
Capable of modeling based on actual individual comeal information.

Benefits

Providing a more comprehensive reference base.
Better predictive accuracy of irritation test outcomes.
Assistance with individualized assessments and favoring customized schemes.

Additional 3D Biology-based Ocular Toxicity Assessment Services

Creative Biolabs with extensive experience utilizing the proven human 3D cornea epithelial model to predict the ocular irritation properties of drugs or formulas. Please do not hesitate to contact us, our seasoned scientists will reply to you rapidly.

Reference

Wan, Xichen et al. "Establishment of human corneal epithelial organoids for ex vivo modelling dry eye disease." Cell proliferatio vol. 57,11 (2024): e13704. Distributed under Open Access license CC BY 4.0, without modification.

Research Model

3D Biology Based Biomarker Discovery