Oral Irritation Evaluation by Human 3D Buccal or Gingival Model

Creative Biolabs offers a 3D buccal or gingival model representing a highly reproducible, non-animal means oral tissue model to assist clients in oral irritation evaluation.

Oral Irritation

As oral care formulations continue to evolve, they are becoming more complex and contain more active ingredients and excipients. These formulations have the potential to be harsher and may pose a greater risk of causing oral irritation in a portion of the population. One of the most common types of oral irritation is tissue desquamation, which occurs when the soft tissue is exposed to certain dentifrice/rinse formulations.

3D Buccal or Gingival Models

	3D gingival tissue model	3D oral mucosa tissue model
Feature	It simulates physiological oxygen tension and reproduces the natural periodontal pocket, allowing the human microbiome to persist with a physiological degree of microbial diversity.	It is histologically comparable to human oral mucosa and has significant transepithelial electrical resistance (TEER).
Work flow	Implant collagen I-embedded human gingival fibroblasts onto the scaffold to imitate connective tissue. Sow human gingival epithelial cells on the scaffold's apical region to mimic epithelial tissue. Implant Dental resin teeth to recreate the periodontal pocket.	Add cell culture insert, medium, human keratinocyte growth supplement to each well of the culture plate. Prepare epithelial cell suspensions and add them to cell culture insert, and add media to the insert. Add medium to each well of the culture plate and remove the medium in the inserts after 24 hours of incubation. Expose the inserts to the air to enable cell differentiation, renew medium in the well every other day, and the inserts were ready for use on day 14.
Case study	Fig.1 Epithelial transmembrane resistance measured by 3D gingival tissue model.¹	Fig.2 Cell Viability after treatment with antiseptic agents and antiseptic rinses with 3D oral mucosa tissue model.²

Advantages of 3D Buccal or Gingival Model Compared to 2D culture

Replication of the differentiated structure and function of native tissues in many aspects.
Simpler to use and more readily accessible.
Suitable for the method of exposure to test substances and/or xenobiotics.
More realistically simulates drug sensitivity and drug metabolism, gene and protein expression, interaction among cells, and other phenomena.
Suitable for the method of exposure to test substances and/or xenobiotics.
Avoidance of interspecies differences between animals and humans, and implementation of extrapolating of animal data.
Possibility of focusing on specific phenomena of interest and interpreting experimental data more easily due to the lower complexity of oral tissue models than whole organism models.
Compliant with EU animal testing legislation, which forbids animal testing of oral care products in the European Union.

Human 3D buccal or gingival models are capable of reproducing the intricate structures of the natural periodontal pocket and human oral mucosa. Creative Biolabs is professional in constructing 3D buccal or gingival models to satisfy your various research on oral irritation. If you have related needs, please contact us for more information.

References

Adelfio, Miryam et al. "Three-Dimensional Humanized Model of the Periodontal Gingival Pocket to Study Oral Microbiome." Advanced science (Germany). 10,12 (2023): e2205473. Distributed under Open Access license CC BY 4.0, the original image's title was changed to "Epithelial transmembrane resistance measured by 3D gingival tissue model".
Zwicker, Paula et al. "A 3D Model of Human Buccal Mucosa for Compatibility Testing of Mouth Rinsing Solutions." Pharmaceutics. 15,3 (2023): 721. Distributed under Open Access license CC BY 4.0, the original image's title was changed to "Cell Viability after treatment with antiseptic agents and antiseptic rinses with 3D oral mucosa tissue model".

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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