Tumor Organoid and CAR-T Co-Culture Model for Immuno-Oncology Drug Discovery and Development

CAR-T cells possess CAR, the chimeric antigen receptor, demonstrating more accurate potency and responding to more realistic tumor eradication outcomes in tumor cell-based organoid models. Creative Biolabs is skilled in providing tumor organoid and CAR-T co-culture models for drug development.

CAR-T Cells Acting in Immuno-oncology

Targeted anti-tumor therapy.

By recognizing and killing specific antigens, CAR-T cells can precisely attack tumor cells, release cytotoxins, activate the killing effect of immune cells, and produce cytokines, etc. This specific targeting gives CAR-T cells a better ability to eliminate tumors.

Immunological assessment.

CAR-T cells can be evaluated for their persistence, expansion capacity, and killing effect to guide and optimize their related immunological intervention strategies.

Personalized medicine.

The patient's own T cells are genetically modified to produce CAR-T cells, enabling customized treatment plans.

Research on immune tolerance.

CAR-T cells may face challenges of immune tolerance when treating some tumors. Studying the mechanisms of immune escape in CAR-T cell immunotherapy is essential for developing therapeutic strategies against immune tolerance.

Fig 1. Establishing CAR-T co-culture system. Fig 1. Preparation process of tumor organoids and CAR-T co-culture basic model.¹

Co-culture Modeling and Applications

Model Building

Applications

Organoid culture. Tumor samples or tumor cell lines from patients are cultured in appropriate cell culture media to form organoid models that mimic the primary tumor.
CAR-T cell preparation. T cells are obtained from patients and genetically modified to express specific CARs.
Co-culture model construction. CAR-T cells are co-cultured with tumor-like organs in the appropriate medium. This co-culture model can simulate the exchange of CAR-T cells with tumor cells in vivo.

Drug Screening. By testing tumor cell survival and CAR-T cell-killing effects in the model, it is possible to screen for drugs with high activity against specific tumors.
Mechanistic studies. Detecting the initiation, proliferation, and cytokine release of CAR-T cells in co-culture models can help to gain insight into the mechanism of CAR-T cells' role in immunotherapy.
Personalized medicine. Based on the individual differentiation of tumor characteristics and immune status, individualized co-culture models are established to provide a basis for the formulation of CAR-T cell intervention platforms.

Fig. 2 CAR-T cells as one of the conditions for cancer organoids co-culture.²

Creative Biolabs provides CAR-T cell and tumor-like organ co-culture models to more realistically simulate the responsiveness of CAR-T cell efficacy and help advance the development of immune therapeutics. Please contact us with your project requirements.

References

Ning, R-X. et al. Application status and optimization suggestions of tumor organoids and CAR-T cell co-culture models. Cancer Cell Int. 2024, 24(1): 98. Distributed under Open Access License CC BY 4.0, without modification.
Yuan, Jin, Xiaoyang Li, and Shengji Yu. "Cancer organoid co-culture model system: Novel approach to guide precision medicine." Frontiers in Immunology 13 (2023): 1061388. Distributed under Open Access License CC BY 4.0, without modification.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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