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Vapaliximab (CSC-Ps0261)

DESCRIPTION CATALOG # SIZE PRICE
Anti-Human AOC3 Stable Cell Line CSC-Ps0261 1×107cells/vial Please Inquiry

PRODUCT INFORMATION

  • Cell Line Description
  • Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity. A pseudogene for this gene has been discribed and is located approximately 9-kb downstream.
  • CAS
  • 336801-86-6
  • Growth Properties
  • Suspension
  • Morphology
  • Epithelial-like
  • Propagation
  • Complete growth medium: Serum-free Medium
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
  • Starting Cells From Frozen Cell Stock
  • 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37°C water bath for 2 minutes until nearly (80%) thawed.
    Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.
    2. Clean the outside of the vial with 70% ethanol before opening.
    3. Remove the cells from the vial and add slowly into a 15ml conical tube containing 10 ml pre-warmed media.
    4. Centrifuge for 3 minutes 1000 xg to pellet cells and remove the supernatant.
    5. Add 14 ml of media and transfer cells to a T25 flask or a 100 mm culture dish.
    6. Place the cells in the 37°C incubator with 5% CO2.
    7. Allow incubation for 3-4 days to reach confluence. The cells will re-attach to the surface over a period of several days in culture at 37°C.
  • Subculturing
  • 1. Centrifuge for 5 minutes 300 xg and discard culture medium.
    2. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    3. Add appropriate aliquots of the cell suspension to new culture vessels.
    Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
    Medium Renewal: 2 to 3 times per week
  • Mycoplasma
  • Mycoplasma Status: Negative (MycoAlert Kit)
  • Freeze Medium
  • Complete growth medium 90%; DMSO, 10%
  • Safety Considerations
  • The following safety precautions should be observed.
    1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
    2. No eating, drinking or smoking while handling the stable line.
    3. Wash hands after handling the stable line and before leaving the lab.
    4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
    5. All waste should be considered hazardous.
    6. Dispose of all liquid waste after each experiment and treat with bleach.
  • Ship
  • Dry ice
  • Product Purity
  • >98.0% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE.
  • Product Type
  • Chimeric (mouse/human) IgG2
  • Product Molecular Mass
  • Approximately 150 kDa
  • Product Storage
  • It should be stored in liquid or vapor phase nitrogen. Reconstituted antibody aliquots should avoid repeated freeze-thaw cycles.
  • Involvement in Disease
  • Vapaliximab is a chimeric monoclonal antibody and an immunosuppressive drug.

BACKGROUND

  • Official Symbol
  • AOC3
  • Synonyms
  • AOC3; amine oxidase, copper containing 3 (vascular adhesion protein 1); membrane primary amine oxidase; HPAO; VAP 1; VAP1; copper amine oxidase; vascular adhesion protein 1; semicarbazide-sensitive amine oxidase; SSAO; VAP-1;
  • UniProt ID
  • Q16853
  • Chromosome Location
  • 17q21
  • Pathway
  • Glycine, serine and threonine metabolism, organism-specific biosystem; Glycine, serine and threonine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Phenylalanine metabolism, organism-specific biosystem; Phenylalanine metabolism, conserved biosystem; Tyrosine metabolism, organism-specific biosystem; Tyrosine metabolism, conserved biosystem;
  • Function
  • aliphatic-amine oxidase activity; aminoacetone:oxygen oxidoreductase(deaminating) activity; calcium ion binding; cation channel activity; copper ion binding; copper ion binding; oxidoreductase activity; phenethylamine:oxygen oxidoreductase (deaminating) activity; primary amine oxidase activity; protein homodimerization activity; quinone binding; tryptamine:oxygen oxidoreductase (deaminating) activity;

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