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Foralumab (CSC-Ps0245)

DESCRIPTION CATALOG # SIZE PRICE
Anti-Human CD3 epsilon Stable Cell Line CSC-Ps0245 1×107cells/vial Please Inquiry

PRODUCT INFORMATION

  • Cell Line Description
  • The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
  • CAS
  • 946415-64-1
  • Growth Properties
  • Suspension
  • Morphology
  • Epithelial-like
  • Propagation
  • Complete growth medium: Serum-free Medium
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
  • Starting Cells From Frozen Cell Stock
  • 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37°C water bath for 2 minutes until nearly (80%) thawed.
    Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.
    2. Clean the outside of the vial with 70% ethanol before opening.
    3. Remove the cells from the vial and add slowly into a 15ml conical tube containing 10 ml pre-warmed media.
    4. Centrifuge for 3 minutes 1000 xg to pellet cells and remove the supernatant.
    5. Add 14 ml of media and transfer cells to a T25 flask or a 100 mm culture dish.
    6. Place the cells in the 37°C incubator with 5% CO2.
    7. Allow incubation for 3-4 days to reach confluence. The cells will re-attach to the surface over a period of several days in culture at 37°C.
  • Subculturing
  • 1. Centrifuge for 5 minutes 300 xg and discard culture medium.
    2. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    3. Add appropriate aliquots of the cell suspension to new culture vessels.
    Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
    Medium Renewal: 2 to 3 times per week
  • Mycoplasma
  • Mycoplasma Status: Negative (MycoAlert Kit)
  • Freeze Medium
  • Complete growth medium 90%; DMSO, 10%
  • Safety Considerations
  • The following safety precautions should be observed.
    1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
    2. No eating, drinking or smoking while handling the stable line.
    3. Wash hands after handling the stable line and before leaving the lab.
    4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
    5. All waste should be considered hazardous.
    6. Dispose of all liquid waste after each experiment and treat with bleach.
  • Ship
  • Dry ice
  • Product Purity
  • >98.0% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE.
  • Product Type
  • Human IgG1 - kappa
  • Product Molecular Mass
  • Approximately 150 kDa
  • Product Storage
  • It should be stored in liquid or vapor phase nitrogen. Reconstituted antibody aliquots should avoid repeated freeze-thaw cycles.
  • Involvement in Disease
  • Foralumab is an immunomodulator. It binds to CD3 epsilon.

BACKGROUND

  • Official Symbol
  • CD3E
  • Synonyms
  • CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683;
  • UniProt ID
  • P07766
  • Chromosome Location
  • 11q23
  • Pathway
  • Adaptive Immune System, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Downstream TCR signaling, organism-specific biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem;
  • Function
  • SH3 domain binding; T cell receptor binding; protein heterodimerization activity; protein kinase binding; receptor activity; receptor signaling complex scaffold activity; receptor signaling protein activity; transmembrane signaling receptor activity;

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