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Gemtuzumab (CSC-Ps0013)

DESCRIPTION CATALOG # SIZE PRICE
Anti-Human CD33 Stable Cell Line (Gemtuzumab) CSC-Ps0013 1×107cells/vial Please Inquiry

PRODUCT INFORMATION

  • Cell Line Description
  • CHO-Anti-Human CD33 Mab Stable Cell Line is clonally-derived from a CHO cell line, which has been transfected with an anti-human CD33 Mab gene to allow expression of the MAb.
  • CAS
  • 220578-59-6
  • Growth Properties
  • Suspension
  • Morphology
  • Epithelial-like
  • Host Cell
  • CHO
  • Propagation
  • Complete growth medium: Serum-free Medium
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 34°C
  • Starting Cells From Frozen Cell Stock
  • 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37 °C water bath for 2 minutes until nearly (80%) thawed.
    Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.
    2. Clean the outside of the vial with 70% ethanol before opening.
    3. Remove the cells from the vial and add slowly into a 15 ml conical tube containing 10 ml pre-warmed media.
    4. Centrifuge for 5 minutes 300 xg to pellet cells and remove the supernatant.
    5. Add 14 ml of media and transfer cells to a T25 shaker flask.
    6. Place the cells in the 37 °C incubator with 5% CO2.
    7. Allow incubation for 3-4 days to reach confluence. The cells will grow well over a period of several days in culture at 37 °C.
  • Subculturing
  • 1. Centrifuge for 5 minutes 300 xg and discard culture medium.
    2. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    3. Add appropriate aliquots of the cell suspension to new culture vessels.
    Incubate cultures at 37 °C.
    Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
    Medium Renewal: 2 to 3 times per week
  • Mycoplasma
  • Mycoplasma Status: Negative (MycoAlert Kit)
  • Freeze Medium
  • Complete growth medium 90%; DMSO, 10%
  • Storage
  • Liquid nitrogen vapor phase
  • Safety Considerations
  • The following safety precautions should be observed.
    1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
    2. No eating, drinking or smoking while handling the stable line.
    3. Wash hands after handling the stable line and before leaving the lab.
    4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
    5. All waste should be considered hazardous.
    6. Dispose of all liquid waste after each experiment and treat with bleach.
  • Ship
  • Dry ice
  • Isotype
  • Humanized IgG4-kappa
  • Product Molecular Mass
  • 151-153kDa
  • Product Species Activity
  • Tested positive against native human antigen.
  • Product Storage
  • It should be stored in liquid or vapor phase nitrogen. Reconstituted antibody aliquots should avoid repeated freeze-thaw cycles.
  • Involvement in Disease
  • Gemtuzumab ozogamicin is a drug-linked monoclonal antibody (an antibody-drug conjugate) that was used to treat acute myelogenous leukemia from 2000-2010.
  • Keywords
  • Gemtuzumab; mylotarg; 220578-59-6; CD33; CD33 molecule; CD33 antigen (gp67); myeloid cell surface antigen CD33; FLJ00391; p67; sialic acid binding Ig like lectin 3; SIGLEC 3; SIGLEC3; gp67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC-3

BACKGROUND

  • Official Symbol
  • CD33
  • Synonyms
  • CD33; CD33 molecule; CD33 antigen (gp67); myeloid cell surface antigen CD33; FLJ00391; p67; sialic acid binding Ig like lectin 3; SIGLEC 3; SIGLEC3; gp67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC-3
  • UniProt ID
  • P20138
  • Chromosome Location
  • 19q13.3
  • Pathway
  • Hematopoietic cell lineage
  • Function
  • receptor activity; sugar binding

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