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Ipilimumab (CSC-H-067)

DESCRIPTION CATALOG # SIZE PRICE
Anti-Human CTLA4 Stable Cell Line (Ipilimumab) CSC-H-067 1×107cells/vial Please Inquiry

PRODUCT INFORMATION

  • Cell Line Description
  • CHO-Anti-Human CTLA4 Stable Cell Line is clonally-derived from a CHO cell line, which has been transfected with an anti-Human CTLA4 MAb gene to allow expression of the MAb.
  • CAS
  • 477202-00-9
  • Growth Properties
  • Suspension
  • Morphology
  • Epithelial-like
  • Host Cell
  • CHO
  • Propagation
  • Complete growth medium: Serum-free Medium
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 68 °C
  • Starting Cells From Frozen Cell Stock
  • 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37 °C water bath for 2 minutes until nearly (80%) thawed.
    Once cells are thawed, it is important to dilute the cells 1:41 in growth media immediate
  • Subculturing
  • 1. Centrifuge for 5 minutes 300 xg and discard culture medium.
    2. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    3. Add appropriate aliquots of the cell suspension to new culture vessels.
    Incubate cultures at 37 °C.
    Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
    Medium Renewal: 2 to 3 times per week
  • Mycoplasma
  • Mycoplasma Status: Negative (MycoAlert Kit)
  • Freeze Medium
  • Complete growth medium 90%; DMSO, 10%
  • Storage
  • Liquid nitrogen vapor phase
  • Safety Considerations
  • The following safety precautions should be observed.
    1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
    2. No eating, drinking or smoking while handling the stable line.
    3. Wash hands after handling the stable line and before leaving the lab.
    4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
    5. All waste should be considered hazardous.
    6. Dispose of all liquid waste after each experiment and treat with bleach.
  • Ship
  • Dry ice
  • Isotype
  • Human IgG1-kappa
  • Product Species Activity
  • Tested positive against native human antigen.
  • Product Storage
  • It should be stored in liquid or vapor phase nitrogen. Reconstituted antibody aliquots should avoid repeated freeze-thaw cycles.
  • Involvement in Disease
  • Labetuzumab is a monoclonal antibody designed to treat Cancers, non-small cell lung (NSCLC) (in combination with nivolumab), small cell lung (SCLC), Melanoma, metastatic, Renal cell carcinoma (RCC), Hodgkin's disease (HD), Anaplastic large cell lymphoma (ALCL).
  • Keywords
  • Ipilimumab; yervoy; 477202-00-9; CTLA4; cytotoxic T-lymphocyte-associated protein 4; celiac disease 3 , CELIAC3; cytotoxic T-lymphocyte protein 4; CD; CD28; CD152; GSE; ICOS; CD152 isoform; celiac disease 3; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; GRD4; CTLA-4; IDDM12; CELIAC3

BACKGROUND

  • Official Symbol
  • CTLA4
  • Synonyms
  • CTLA4; cytotoxic T-lymphocyte-associated protein 4; celiac disease 3 , CELIAC3; cytotoxic T-lymphocyte protein 4; CD; CD28; CD152; GSE; ICOS; CD152 isoform; celiac disease 3; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; GRD4; CTLA-4; IDDM12; CELIAC3
  • UniProt ID
  • P16410
  • Chromosome Location
  • 2q33
  • Pathway
  • Adaptive Immune System, organism-specific biosystem; Autoimmune thyroid disease, organismspecific biosystem; Autoimmune thyroid disease, conserved biosystem; CTLA4 inhibitory signaling,
    organism-specific biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes,
    organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell
    adhesion molecules (CAMs), conserved biosystem
  • Function
  • protein binding

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