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Mepolizumab (CSC-P0122)

DESCRIPTION CATALOG # SIZE PRICE
Anti-Human IL5 Stable Cell Line (Mepolizumab) CSC-P0122 1×107cells/vial Please Inquiry

PRODUCT INFORMATION

  • Cell Line Description
  • CHO-Anti-Human IL5 Stable Cell Line is clonally-derived from a CHO cell line, which has been transfected with an anti-Human IL5 MAb gene to allow expression of the MAb.
  • CAS
  • 196078-29-2
  • Growth Properties
  • Suspension
  • Morphology
  • Epithelial-like
  • Host Cell
  • CHO
  • Propagation
  • Complete growth medium: Serum-free Medium
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 68 °C
  • Starting Cells From Frozen Cell Stock
  • 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37 °C water bath for 2 minutes until nearly (80%) thawed.
    Once cells are thawed, it is important to dilute the cells 1:41 in growth media immediate
  • Subculturing
  • 1. Centrifuge for 5 minutes 300 xg and discard culture medium.
    2. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    3. Add appropriate aliquots of the cell suspension to new culture vessels.
    Incubate cultures at 37 °C.
    Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
    Medium Renewal: 2 to 3 times per week
  • Mycoplasma
  • Mycoplasma Status: Negative (MycoAlert Kit)
  • Freeze Medium
  • Complete growth medium 90%; DMSO, 10%
  • Storage
  • Liquid nitrogen vapor phase
  • Safety Considerations
  • The following safety precautions should be observed.
    1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
    2. No eating, drinking or smoking while handling the stable line.
    3. Wash hands after handling the stable line and before leaving the lab.
    4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
    5. All waste should be considered hazardous.
    6. Dispose of all liquid waste after each experiment and treat with bleach.
  • Ship
  • Dry ice
  • Isotype
  • Humanized (from mouse) IgG1-kappa
  • Product Species Activity
  • Tested positive against native human antigen.
  • Product Storage
  • It should be stored in liquid or vapor phase nitrogen. Reconstituted antibody aliquots should avoid repeated freeze-thaw cycles.
  • Involvement in Disease
  • Mepolizumab is a humanized monoclonal antibody that recognizes interleukin-5 (IL-5), and is used to treat certain kinds of asthma and white blood cell diseases. Recent studies have concluded that mepolizumab may improve exacerbations in patients with severe eosinophilic asthma, an adult-onset asthma which represents less than 5% of all asthma. IL-5 is a chemical messenger in the immune system that stimulates the growth of eosinophils. In eosinophilic asthma, eosinophils are present in the lungs. When mepolizumab was given to people with eosinophilic asthma, it eliminated eosinophils from the bloodstream,and reduced eosinophils in the lungs and bone marrow. Mepolizumab also reduced the number of asthma exacerbations, and reduced the need for corticosteroids.
  • Keywords
  • Mepolizumab; Bosatria; 196078-29-2; IL5; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; B cell differentiation factor I; EDF; eosinophil differentiation factor; IL 5; interleukin 5; T cell replacing factor; TRF; T-cell replacing factor; B-cell differentiation factor I; IL-5

BACKGROUND

  • Official Symbol
  • IL5
  • Synonyms
  • IL5; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; B cell differentiation factor I; EDF; eosinophil differentiation factor; IL 5; interleukin 5; T cell replacing factor; TRF; T-cell replacing factor; B-cell differentiation factor I; IL-5
  • UniProt ID
  • P05113
  • Chromosome Location
  • 5q23-q31
  • Pathway
  • Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma,
    organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organismspecific biosystem; Autoimmune thyroid disease, conserved biosystem; Calcineurin-regulated NFATdependent transcription in lymphocytes, organism-specific biosystem
  • Function
  • cytokine activity; growth factor activity; interleukin-5 receptor binding; protein binding

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