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CHO-Anti-Human IL5 Stable Cell Line is clonally-derived from a CHO cell line, which has been transfected with an anti-Human IL5 MAb gene to allow expression of the MAb.
Complete growth medium: Serum-free Medium Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 68 °C
Starting Cells From Frozen Cell Stock
1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37 °C water bath for 2 minutes until nearly (80%) thawed. Once cells are thawed, it is important to dilute the cells 1:41 in growth media immediate
1. Centrifuge for 5 minutes 300 xg and discard culture medium. 2. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 3. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37 °C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week
Mycoplasma Status: Negative (MycoAlert Kit)
Complete growth medium 90%; DMSO, 10%
Liquid nitrogen vapor phase
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Humanized (from rat) IgG4
Product Species Activity
Tested positive against native human antigen.
It should be stored in liquid or vapor phase nitrogen. Reconstituted antibody aliquots should avoid repeated freeze-thaw cycles.
Involvement in Disease
Reslizumab is a humanized monoclonal antibody intended for the treatment of eosinophil-meditated inflammations of the airways, skin and gastrointestinal tract.