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Anthrax toxins are composed of three distinct proteins, a protective antigen (PA), a lethal factor (LF) and an edema factor (EF). None of these proteins are toxic by themselves and several studies indicate that the anthrax toxin has the familiar A-B enzymatic binding structure, with PA acting as the binding domain and EF and/or LF acting as the active fragments.
1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37°C water bath for 2 minutes until nearly (80%) thawed. Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells. 2. Clean the outside of the vial with 70% ethanol before opening. 3. Remove the cells from the vial and add slowly into a 15ml conical tube containing 10 ml pre-warmed media. 4. Centrifuge for 3 minutes 1000 xg to pellet cells and remove the supernatant. 5. Add 14 ml of media and transfer cells to a T25 flask or a 100 mm culture dish. 6. Place the cells in the 37°C incubator with 5% CO2. 7. Allow incubation for 3-4 days to reach confluence. The cells will re-attach to the surface over a period of several days in culture at 37°C.
1. Centrifuge for 5 minutes 300 xg and discard culture medium. 2. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 3. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week
Mycoplasma Status: Negative (MycoAlert Kit)
Complete growth medium 90%; DMSO, 10%
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
HPLC > 98%, One strip for SDS-PAGE.
Humanized (from mouse) IgG1
Product Molecular Mass
It should be stored in liquid or vapor phase nitrogen. Reconstituted antibody aliquots should avoid repeated freeze-thaw cycles.
Involvement in Disease
Talizumab is a humanized monoclonal antibody as a new-concept therapeutic for allergic diseases. The unique anti-IgE antibody was designed to target immunoglobulin E (IgE) and IgE-expressing B lymphocytes specifically, without binding to IgE already bound by the high affinity IgE receptors on mast cells and basophils.
Immunoglobulin E (IgE) is a class of antibody (or immunoglobulin (Ig) "isotype") that has been found only in mammals. IgE exists as monomers consisting of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgE's main function is immunity to parasites such as parasitic worms like Schistosoma mansoni, Trichinella spiralis, and Fasciola hepatica. IgE may also be important during immune defense against certain protozoan parasites such as Plasmodium falciparum.
IgE Fc; IgE; Immunoglobulin E; Immunoglobulin E Fc