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Denosumab (CSC-H-H18)

DESCRIPTION CATALOG # SIZE PRICE
Anti-Human TNFSF11 Stable Cell Line (denosumab) CSC-H-H18 1×107cells/vial Please Inquiry

PRODUCT INFORMATION

  • Cell Line Description
  • CHO-Anti-Human TNFSF11 Stable Cell Line is clonally-derived from a CHO cell line, which has been transfected with an anti-Human TNFSF11 MAb gene to allow expression of the MAb.
  • CAS
  • 615258-40-7
  • Growth Properties
  • Suspension
  • Morphology
  • Epithelial-like
  • Host Cell
  • CHO
  • Propagation
  • Complete growth medium: Serum-free Medium
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 68 °C
  • Starting Cells From Frozen Cell Stock
  • 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37 °C water bath for 2 minutes until nearly (80%) thawed.
    Once cells are thawed, it is important to dilute the cells 1:41 in growth media immediate
  • Subculturing
  • 1. Centrifuge for 5 minutes 300 xg and discard culture medium.
    2. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    3. Add appropriate aliquots of the cell suspension to new culture vessels.
    Incubate cultures at 37 °C.
    Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
    Medium Renewal: 2 to 3 times per week
  • Mycoplasma
  • Mycoplasma Status: Negative (MycoAlert Kit)
  • Freeze Medium
  • Complete growth medium 90%; DMSO, 10%
  • Storage
  • Liquid nitrogen vapor phase
  • Safety Considerations
  • The following safety precautions should be observed.
    1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
    2. No eating, drinking or smoking while handling the stable line.
    3. Wash hands after handling the stable line and before leaving the lab.
    4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
    5. All waste should be considered hazardous.
    6. Dispose of all liquid waste after each experiment and treat with bleach.
  • Ship
  • Dry ice
  • Isotype
  • Human IgG2-kappa
  • Product Molecular Mass
  • Approximately 148 kDa
  • Product Species Activity
  • Tested positive against native human antigen.
  • Product Storage
  • It should be stored in liquid or vapor phase nitrogen. Reconstituted antibody aliquots should avoid repeated freeze-thaw cycles.
  • Keywords
  • denosumab; xgeva; 615258-40-7; TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11; CD254; ODF; OPGL; RANKL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; sOdf; OPTB2; hRANKL2

BACKGROUND

  • Official Symbol
  • TNFSF11
  • Synonyms
  • TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11; CD254; ODF; OPGL; RANKL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; sOdf; OPTB2; hRANKL2
  • UniProt ID
  • O14788
  • Chromosome Location
  • 13q14
  • Pathway
  • Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor
    interaction, conserved biosystem; IL6-mediated signaling events, organism-specific biosystem;
    Osteoblast Signaling, organism-specific biosystem; Osteoclast Signaling, organism-specific biosystem;
    Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved
    biosystem;
  • Function
  • cytokine activity; cytokine activity; receptor activity; tumor necrosis factor receptor binding; tumor necrosis factor receptor superfamily binding

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