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Belimumab (CSC-P0221)

DESCRIPTION CATALOG # SIZE PRICE
Anti-Human TNFSF13B Stable Cell Line (Belimumab) CSC-P0221 1×107cells/vial Please Inquiry

PRODUCT INFORMATION

  • Cell Line Description
  • CHO-Anti-TNFSF13B Stable Cell Line is clonally-derived from a CHO cell line, which has been transfected with an anti-Human BLys MAb gene to allow expression of the MAb.
  • CAS
  • 356547-88-1
  • Growth Properties
  • Suspension
  • Morphology
  • Epithelial-like
  • Host Cell
  • CHO
  • Propagation
  • Complete growth medium: Serum-free Medium
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 68 °C
  • Starting Cells From Frozen Cell Stock
  • 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37 °C water bath for 2 minutes until nearly (80%) thawed.
    Once cells are thawed, it is important to dilute the cells 1:41 in growth media immediate
  • Subculturing
  • 1. Centrifuge for 5 minutes 300 xg and discard culture medium.
    2. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    3. Add appropriate aliquots of the cell suspension to new culture vessels.
    Incubate cultures at 37 °C.
    Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
    Medium Renewal: 2 to 3 times per week
  • Mycoplasma
  • Mycoplasma Status: Negative (MycoAlert Kit)
  • Freeze Medium
  • Complete growth medium 90%; DMSO, 10%
  • Storage
  • Liquid nitrogen vapor phase
  • Safety Considerations
  • The following safety precautions should be observed.
    1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
    2. No eating, drinking or smoking while handling the stable line.
    3. Wash hands after handling the stable line and before leaving the lab.
    4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
    5. All waste should be considered hazardous.
    6. Dispose of all liquid waste after each experiment and treat with bleach.
  • Ship
  • Dry ice
  • Isotype
  • Human IgG1-lambda
  • Product Molecular Mass
  • Approximately 150 kDa
  • Product Species Activity
  • Tested positive against native human antigen.
  • Product Storage
  • It should be stored in liquid or vapor phase nitrogen. Reconstituted antibody aliquots should avoid repeated freeze-thaw cycles.
  • Involvement in Disease
  • Belimumab is a human monoclonal antibody that inhibits B-cell activating factor (BAFF), lso known as B-lymphocyte stimulator (BLyS). B cells are responsible for part of the normal immune response, and also for the over-aggressive immune response in autoimmune diseases like systemic lupus erythematosus (SLE).
  • Keywords
  • Belimumab; LymphoStat-B; hBlySmAb-1.1; BENLYSTA; 356547-88-1; TNFSF13B; tumor necrosis factor (ligand) superfamily, member 13b; TNFSF20; tumor necrosis factor ligand superfamily member 13B; BAFF; BLYS; CD257; TALL 1; TNFSF13B; tumor necrosis factor (ligand) superfamily, member 13b; TNFSF20; tumor necrosis factor ligand superfamily member 13B; BAFF; BLYS; CD257; TALL 1; TALL1; THANK; delta BAFF; Delta4 BAFF; B-lymphocyte stimulator; B-cell-activating factor; ApoL related ligand TALL-1; TNF homolog that activates apoptosis; dendritic cell-derived TNF-like molecule; tumor necrosis factor-like protein ZTNF4; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily, member 20; DTL; ZTNF4; TALL-1

BACKGROUND

  • Official Symbol
  • TNFSF13B
  • Synonyms
  • TNFSF13B; tumor necrosis factor (ligand) superfamily, member 13b; TNFSF20; tumor necrosis factor ligand superfamily member 13B; BAFF; BLYS; CD257; TALL 1; TALL1; THANK; delta BAFF; Delta4 BAFF; B-lymphocyte stimulator; B-cell-activating factor; ApoL related ligand TALL-1; TNF homolog that activates apoptosis; dendritic cell-derived TNF-like molecule; tumor necrosis factor-like protein ZTNF4; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily, member 20; DTL; ZTNF4; TALL-1
  • UniProt ID
  • Q9Y275
  • Chromosome Location
  • 13q32-q34
  • Pathway
  • Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor
    interaction, conserved biosystem; Intestinal immune network for IgA production, organism-specific
    biosystem; Intestinal immune network for IgA production, conserved biosystem; Rheumatoid arthritis,
    organism-specific biosystem; Rheumatoid arthritis, conserved biosystem
  • Function
  • cytokine activity; protein binding; receptor binding; tumor necrosis factor receptor binding

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